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Name - Podocyte Nested FFM model (copy sensitivity)

BioModel ID - 83091496

Reaction Diagram

Physiology

Universal ID's	Text Annotations

Reactions See in Dashboard
Reaction	Name Structure	Kinetics	Depiction
Gs_a_PM+AC_PMAC_Gsa_PM	04: Gs-AC binding PM	GeneralKinetics	Gs_a_PM+AC_PMAC_Gsa_PM
		reaction rate (J): ((AC_PM * Kf_04__Gs_AC_binding * Gs_a_PM) - (Kr_04__Gs_AC_binding * AC_Gsa_PM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] user defined (Kf_04__Gs_AC_binding): 1.0[um2.molecules-1.s-1] user defined (Kr_04__Gs_AC_binding): 3.4[s-1]
ATP_cellcAMP_cell	05b: cAMP production PM	HMMIrreversible	ATP_cellcAMP_cell
		reaction rate (J): ((Vmax * ATP_cell) / (Km + ATP_cell))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] Km (1/2 max) (Km): 3.15[uM] max reaction rate (Vmax): (kcat * AC_Gsa_PM)[molecules.um-2.s-1] user defined (kcat): 8.5[s-1]
R2C2_cell+cAMP_cellcAMP_R2C2_cell	cAMP bind R2C2 cell	MassAction	R2C2_cell+cAMP_cellcAMP_R2C2_cell
		reaction rate (J): (((Kf * R2C2_cell) * cAMP_cell) - (Kr * cAMP_R2C2_cell))[uM.s-1] forward rate constant (Kf): 54.0[s-1.uM-1] reverse rate constant (Kr): 33.0[s-1]
MAPK_active_nucleusMAPK_thr_nucleus	MKP2 deact MAPKact nucleus	HMMIrreversible	MAPK_active_nucleusMAPK_thr_nucleus
		reaction rate (J): ((Vmax * MAPK_active_nucleus) / (Km + MAPK_active_nucleus))[uM.s-1] Km (1/2 max) (Km): 0.0667[uM] max reaction rate (Vmax): (kcat * MKP2_nucleus)[uM.s-1] user defined (kcat): 1.0[s-1]
Rap1GAP_cell+Rap1_GTP_GMRap1_RGII_GM	10a: Rap1-RGII binding GM	MassAction	Rap1GAP_cell+Rap1_GTP_GMRap1_RGII_GM
		reaction rate (J): (((Kf_10a__Rap1_RGII_binding * Rap1GAP_cell) * Rap1_GTP_GM) - (Kr_10a__Rap1_RGII_binding * Rap1_RGII_GM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf_10a__Rap1_RGII_binding): 0.62[s-1.uM-1] reverse rate constant (Kr_10a__Rap1_RGII_binding): 8.7[s-1]
cAMP_R2C2_cell+cAMP_cellcAMP2_R2C2_cell	cAMP_binds_cAMPR2C2 cell	MassAction	cAMP_R2C2_cell+cAMP_cellcAMP2_R2C2_cell
		reaction rate (J): (((Kf * cAMP_R2C2_cell) * cAMP_cell) - (Kr * cAMP2_R2C2_cell))[uM.s-1] forward rate constant (Kf): 54.0[s-1.uM-1] reverse rate constant (Kr): 33.0[s-1]
Gs_a_PMGsa_GDP_PM	Gs GTPase PM	GeneralKinetics	Gs_a_PMGsa_GDP_PM
		reaction rate (J): (Gs_a_PM * Kcat)[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] user defined (Kcat): 0.11[s-1]
Rap1_RGII_GMRap1_GDP_GM+Rap1GAP_cell	10: Rap1 GTPase GM	GeneralKinetics	Rap1_RGII_GMRap1_GDP_GM+Rap1GAP_cell
		reaction rate (J_10__Rap1_GTPase): (kcat * Rap1_RGII_GM)[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] user defined (kcat): 8.0[s-1]
MAPKK_Ser_cellMAPKK_cell	PP2A_inact_MAPKKSer cell	HMMIrreversible	MAPKK_Ser_cellMAPKK_cell
		reaction rate (J): ((Vmax * MAPKK_Ser_cell) / (Km + MAPKK_Ser_cell))[uM.s-1] Km (1/2 max) (Km): 15.66[uM] max reaction rate (Vmax): (kcat * PP2A_cell)[uM.s-1] user defined (kcat): 6.0[s-1]
MAPK_cellMAPK_Thr_cell	MAPKK_act_MAPK cell	HMMIrreversible	MAPK_cellMAPK_Thr_cell
		reaction rate (J): ((Vmax * MAPK_cell) / (Km + MAPK_cell))[uM.s-1] Km (1/2 max) (Km): 0.046296[uM] max reaction rate (Vmax): (kcat * MAPKK_active_cell)[uM.s-1] user defined (kcat): 0.15[s-1]
MKP1_RNA_cellMKP1_cell	MKP_translation cell	MassAction	MKP1_RNA_cellMKP1_cell
		reaction rate (J): ((Kf * MKP1_RNA_cell) - (Kr * MKP1_cell))[uM.s-1] forward rate constant (Kf): 0.004[s-1] reverse rate constant (Kr): 0.0[s-1]
MAPKK_Ser_cellMAPKK_active_cell	reaction1 cell	GeneralKinetics	MAPKK_Ser_cellMAPKK_active_cell
		reaction rate (J): (Vmax * MAPKK_Ser_cell / (Km + MAPKK_Ser_cell))[uM.s-1] user defined (Km): 0.159[uM] user defined (Vmax): (kcat * BRaf_star_cell)[uM.s-1] user defined (kcat): 0.105[s-1]
PDE_star_cellPDE_cell	PP2A_dephos_PDEstar cell	HMMIrreversible	PDE_star_cellPDE_cell
		reaction rate (J): ((Vmax * PDE_star_cell) / (Km + PDE_star_cell))[uM.s-1] Km (1/2 max) (Km): 0.96[uM] max reaction rate (Vmax): (kcat * PDE_PP_cell)[uM.s-1] user defined (kcat): 0.77[s-1]
GRK_Gbg_PMGRK_PM+G_bg_PM	GRK PM recruitment PM	MassAction	GRK_Gbg_PMGRK_PM+G_bg_PM
		reaction rate (J): ((Kf_GRK_PM_recruitment * GRK_Gbg_PM) - ((Kr_GRK_PM_recruitment * GRK_PM) * G_bg_PM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf_GRK_PM_recruitment): 1.0[s-1] reverse rate constant (Kr_GRK_PM_recruitment): 1.0E-4[um2.s-1.molecules-1]
PKI_nucleus+PKA_nucleusinhibitedPKA_nucleus	PKA inhibition by PKI nucleus	MassAction	PKI_nucleus+PKA_nucleusinhibitedPKA_nucleus
		reaction rate (J): (((Kf * PKI_nucleus) * PKA_nucleus) - (Kr * inhibitedPKA_nucleus))[uM.s-1] forward rate constant (Kf): 1.0[s-1.uM-1] reverse rate constant (Kr): 1.0E-4[s-1]
cAMP_cell+cAMP2_R2C2_cellcAMP3_R2C2_cell	cAMP_bind_cAMP2R2C2 cell	MassAction	cAMP_cell+cAMP2_R2C2_cellcAMP3_R2C2_cell
		reaction rate (J): (((Kf * cAMP_cell) * cAMP2_R2C2_cell) - (Kr * cAMP3_R2C2_cell))[uM.s-1] forward rate constant (Kf): 75.0[s-1.uM-1] reverse rate constant (Kr): 110.0[s-1]
CREB_Ser_nucleusCREB_nucleus	Pp2A_deact_CREB nucleus	HMMIrreversible	CREB_Ser_nucleusCREB_nucleus
		reaction rate (J): ((Vmax * CREB_Ser_nucleus) / (Km + CREB_Ser_nucleus))[uM.s-1] Km (1/2 max) (Km): 15.66[uM] max reaction rate (Vmax): (kcat * pp2a_nucleus)[uM.s-1] user defined (kcat): 6.0[s-1]
beta_isop_PM+Gs_trimer_PMbetaAR_Gs_PM	2: ARbeta-Gs binding PM	MassAction	beta_isop_PM+Gs_trimer_PMbetaAR_Gs_PM
		reaction rate (J): (((Kf_2__ARbeta_Gs_binding * beta_isop_PM) * Gs_trimer_PM) - (Kr_2__ARbeta_Gs_binding * betaAR_Gs_PM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf_2__ARbeta_Gs_binding): 0.01[um2.s-1.molecules-1] reverse rate constant (Kr_2__ARbeta_Gs_binding): 0.1[s-1]
MKP1gene_nucleusMKP1_RNA_nucleus	MAPK_act_MKPtranscription nucleus	HMMIrreversible	MKP1gene_nucleusMKP1_RNA_nucleus
		reaction rate (J): ((Vmax * MKP1gene_nucleus) / (Km + MKP1gene_nucleus))[uM.s-1] Km (1/2 max) (Km): 4.0[uM] max reaction rate (Vmax): (kcat * MAPK_active_nucleus)[uM.s-1] user defined (kcat): 3.0E-4[s-1]
cAMP_cellAMP_cell	PDEstar_deact_cAMP cell	HMMIrreversible	cAMP_cellAMP_cell
		reaction rate (J): ((Vmax * cAMP_cell) / (Km + cAMP_cell))[uM.s-1] Km (1/2 max) (Km): 1.3[uM] max reaction rate (Vmax): (kcat * PDE_star_cell)[uM.s-1] user defined (kcat): 20.0[s-1]
beta_isop_PMbeta_P_PM	03: GRK activity PM	GeneralKinetics	beta_isop_PMbeta_P_PM
		reaction rate (J_3__GRK_activity): ((beta_isop_PM * GRK_PM * kCATgrk / (KM + beta_isop_PM)) - (beta_P_PM * kdephos))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] user defined (KM): 250.0[molecules.um-2] user defined (kdephos): 0.0[s-1] user defined (kCATgrk): 0.204[s-1]
cAMP4_R2C_cellcAMP4_R2_cell+PKA_cell	Release of C2 cell	MassAction	cAMP4_R2C_cellcAMP4_R2_cell+PKA_cell
		reaction rate (J): ((Kf * cAMP4_R2C_cell) - ((Kr * cAMP4_R2_cell) * PKA_cell))[uM.s-1] forward rate constant (Kf): 0.6[s-1] reverse rate constant (Kr): 0.18[s-1.uM-1]
MKP1gene_nucleusMKP1_RNA_nucleus	MKP1 basal transcription nucleus	MassAction	MKP1gene_nucleusMKP1_RNA_nucleus
		reaction rate (J): ((Kf * MKP1gene_nucleus) - (Kr * MKP1_RNA_nucleus))[uM.s-1] forward rate constant (Kf): 2.0E-6[s-1] reverse rate constant (Kr): 0.0[s-1]
MAPK_Thr_cellMAPK_cell	reaction0 cell	HMMIrreversible	MAPK_Thr_cellMAPK_cell
		reaction rate (J): ((Vmax * MAPK_Thr_cell) / (Km + MAPK_Thr_cell))[uM.s-1] Km (1/2 max) (Km): 0.06667[uM] max reaction rate (Vmax): MKP2_cell[uM.s-1]
MAPK_active_cellMAPK_Thr_cell	reaction3 cell	HMMIrreversible	MAPK_active_cellMAPK_Thr_cell
		reaction rate (J): ((Vmax * MAPK_active_cell) / (Km + MAPK_active_cell))[uM.s-1] Km (1/2 max) (Km): 0.06667[uM] max reaction rate (Vmax): MKP2_cell[uM.s-1]
MAPK_Thr_cellMAPK_cell	MKP_decat_MAPKtyr cell	HMMIrreversible	MAPK_Thr_cellMAPK_cell
		reaction rate (J): ((Vmax * MAPK_Thr_cell) / (Km + MAPK_Thr_cell))[uM.s-1] Km (1/2 max) (Km): 0.067[uM] max reaction rate (Vmax): (kcat * MKP1_cell)[uM.s-1] user defined (kcat): 1.0[s-1]
MAPKK_Ser_cellMAPKK_active_cell	RapRaf_act_MAPKKSer GM	HMMIrreversible	MAPKK_Ser_cellMAPKK_active_cell
		reaction rate (J): ((Vmax * MAPKK_Ser_cell) / (Km + MAPKK_Ser_cell))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] Km (1/2 max) (Km): 0.4[uM] max reaction rate (Vmax): (kcat * Rap1_BRaf_GM)[molecules.um-2.s-1] user defined (kcat): 0.95[s-1]
MKP1p_cell	MKP1p turnover cell	MassAction	MKP1p_cell
		reaction rate (J): (Kf * MKP1p_cell)[uM.s-1] forward rate constant (Kf): 1.0E-4[s-1] reverse rate constant (Kr): 0.0[uM.s-1]
MKP1p_cellMKP1_cell	MKP1 dephosph cell	MassAction	MKP1p_cellMKP1_cell
		reaction rate (J): ((Kf * MKP1p_cell) - (Kr * MKP1_cell))[uM.s-1] forward rate constant (Kf): 1.0E-4[s-1] reverse rate constant (Kr): 0.0[s-1]
MAPK_active_cellMAPK_Thr_cell	MKP1p_deact_MAPKactive cell	HMMIrreversible	MAPK_active_cellMAPK_Thr_cell
		reaction rate (J): ((Vmax * MAPK_active_cell) / (Km + MAPK_active_cell))[uM.s-1] Km (1/2 max) (Km): 0.0667[uM] max reaction rate (Vmax): (kcat * MKP1p_cell)[uM.s-1] user defined (kcat): 1.0[s-1]
betaAR_Gs_PMbeta_isop_PM+Gs_a_PM+G_bg_PM	02b: Gs activation PM	MassAction	betaAR_Gs_PMbeta_isop_PM+Gs_a_PM+G_bg_PM
		reaction rate (J): ((Kf * betaAR_Gs_PM) - (((Kr * beta_isop_PM) * Gs_a_PM) * G_bg_PM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf): 0.025[s-1] reverse rate constant (Kr): 0.0[um4.s-1.molecules-2]
BRaf_cell+Rap1_GTP_GMRap1_BRaf_GM	Rap1_binds_BRaf GM	MassAction	BRaf_cell+Rap1_GTP_GMRap1_BRaf_GM
		reaction rate (J): (((Kf * BRaf_cell) * Rap1_GTP_GM) - (Kr * Rap1_BRaf_GM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf): 4.0[s-1.uM-1] reverse rate constant (Kr): 10.0[s-1]
MAPKK_cellMAPKK_Ser_cell	BRaf_act_MAPKK cell	HMMIrreversible	MAPKK_cellMAPKK_Ser_cell
		reaction rate (J): ((Vmax * MAPKK_cell) / (Km + MAPKK_cell))[uM.s-1] Km (1/2 max) (Km): 0.159[uM] max reaction rate (Vmax): (kcat * BRaf_star_cell)[uM.s-1] user defined (kcat): 0.105[s-1]
BRaf_star_cellBRaf_cell	PP_deact_BRaf cell	HMMIrreversible	BRaf_star_cellBRaf_cell
		reaction rate (J): ((Vmax * BRaf_star_cell) / (Km + BRaf_star_cell))[uM.s-1] Km (1/2 max) (Km): 15.66[uM] max reaction rate (Vmax): (kcat * PP2A_cell)[uM.s-1] user defined (kcat): 6.0[s-1]
BRaf_cellBRaf_star_cell	PKA_act_BRaf cell	GeneralKinetics	BRaf_cellBRaf_star_cell
		reaction rate (J): (Vmax * BRaf_cell / (Km + BRaf_cell))[uM.s-1] user defined (Km): 1.0[uM] user defined (Vmax): (kcat * PKA_cell)[uM.s-1] user defined (kcat): 0.1[s-1]
cAMP3_R2C2_cell+cAMP_cellcAMP4_R2C2_cell	cAMP_binds_A2 cell	MassAction	cAMP3_R2C2_cell+cAMP_cellcAMP4_R2C2_cell
		reaction rate (J): (((Kf * cAMP3_R2C2_cell) * cAMP_cell) - (Kr * cAMP4_R2C2_cell))[uM.s-1] forward rate constant (Kf): 75.0[s-1.uM-1] reverse rate constant (Kr): 32.5[s-1]
GRK_PMGRK_cell	GRK to cytosol PM	MassAction	GRK_PMGRK_cell
		reaction rate (J): ((Kf * GRK_PM) - (Kr * GRK_cell))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf): 0.01[s-1] reverse rate constant (Kr): 0.0[molecules.um-2.s-1.uM-1]
Rap1_Epac_GMEpac_cAMP_cell+Rap1_GTP_GM	08: Epac exchange GM	GeneralKinetics	Rap1_Epac_GMEpac_cAMP_cell+Rap1_GTP_GM
		reaction rate (J_08__Epac_exchange): (Rap1_Epac_GM * kEpacEXCH)[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] user defined (kEpacEXCH): 0.2[s-1]
cAMP4_R2C2_cellcAMP4_R2C_cell+PKA_cell	Release of C1 cell	MassAction	cAMP4_R2C2_cellcAMP4_R2C_cell+PKA_cell
		reaction rate (J): ((Kf * cAMP4_R2C2_cell) - ((Kr * cAMP4_R2C_cell) * PKA_cell))[uM.s-1] forward rate constant (Kf): 0.6[s-1] reverse rate constant (Kr): 0.18[s-1.uM-1]
MAPKK_cellMAPKK_Ser_cell	Rap1raf_act_MAPKK GM	GeneralKinetics	MAPKK_cellMAPKK_Ser_cell
		reaction rate (J): (Vmax * MAPKK_cell / (Km + MAPKK_cell))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] user defined (Km): 0.4[uM] user defined (Vmax): (kcat * Rap1_BRaf_GM)[molecules.um-2.s-1] user defined (kcat): 0.95[s-1]
MKP1_cellMKP1p_cell	MKP1 phospho by MAPKactive cell	HMMIrreversible	MKP1_cellMKP1p_cell
		reaction rate (J): ((Vmax * MKP1_cell) / (Km + MKP1_cell))[uM.s-1] Km (1/2 max) (Km): 25.641[uM] max reaction rate (Vmax): (kcat * MAPK_active_cell)[uM.s-1] user defined (kcat): 1.0[s-1]
MKP1p_nucleus	MKP1p turnover nucleus nucleus	MassAction	MKP1p_nucleus
		reaction rate (J): (Kf * MKP1p_nucleus)[uM.s-1] forward rate constant (Kf): 1.0E-4[s-1] reverse rate constant (Kr): 0.0[uM.s-1]
cAMP_cellAMP_cell	07: PDE cell	HMMIrreversible	cAMP_cellAMP_cell
		reaction rate (J): ((Vmax_07__PDE * cAMP_cell) / (Km_07__PDE + cAMP_cell))[uM.s-1] Km (1/2 max) (Km_07__PDE): 1.3[uM] max reaction rate (Vmax_07__PDE): (kCATpde * PDE_cell)[uM.s-1] user defined (kCATpde): 8.0[s-1]
MAPK_active_nucleusMAPK_thr_nucleus	MKP1p deact MAPKact nucleus	HMMIrreversible	MAPK_active_nucleusMAPK_thr_nucleus
		reaction rate (J): ((Vmax * MAPK_active_nucleus) / (Km + MAPK_active_nucleus))[uM.s-1] Km (1/2 max) (Km): 0.0667[uM] max reaction rate (Vmax): (kcat * MKP1p_nucleus)[uM.s-1] user defined (kcat): 1.0[s-1]
MKP1_nucleus	MKP1 turnover in nucleus nucleus	MassAction	MKP1_nucleus
		reaction rate (J): (Kf * MKP1_nucleus)[uM.s-1] forward rate constant (Kf): 3.7E-4[s-1] reverse rate constant (Kr): 0.0[uM.s-1]
isoprenaline_ec+beta_PMbeta_isop_PM	01b: isoprenaline binding PM	MassAction	isoprenaline_ec+beta_PMbeta_isop_PM
		reaction rate (J): (((Kf_01b__isoprenaline_binding * isoprenaline_ec) * beta_PM) - (Kr_01b__isoprenaline_binding * beta_isop_PM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf_01b__isoprenaline_binding): 0.1[s-1.uM-1] reverse rate constant (Kr_01b__isoprenaline_binding): 0.1[s-1]
PTPSL_star_cellPTPSL_cell	PTPSL_dephos cell	HMMIrreversible	PTPSL_star_cellPTPSL_cell
		reaction rate (J): ((Vmax * PTPSL_star_cell) / (Km + PTPSL_star_cell))[uM.s-1] Km (1/2 max) (Km): 6.0[uM] max reaction rate (Vmax): (kcat * PP1_cell)[uM.s-1] user defined (kcat): 5.0[s-1]
MAPK_Thr_cellMAPK_active_cell	MAPKK_act_MAPK_thr cell	HMMIrreversible	MAPK_Thr_cellMAPK_active_cell
		reaction rate (J): ((Vmax * MAPK_Thr_cell) / (Km + MAPK_Thr_cell))[uM.s-1] Km (1/2 max) (Km): 0.046296[uM] max reaction rate (Vmax): (kcat * MAPKK_active_cell)[uM.s-1] user defined (kcat): 0.15[s-1]
beta_P_PMbeta_PM	Receptor recycling PM	MassAction	beta_P_PMbeta_PM
		reaction rate (J): ((Kf * beta_P_PM) - (Kr * beta_PM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf): 4.7E-4[s-1] reverse rate constant (Kr): 0.0[s-1]
PDE_cellPDE_star_cell	PKA_act_PDE cell	HMMIrreversible	PDE_cellPDE_star_cell
		reaction rate (J): ((Vmax * PDE_cell) / (Km + PDE_cell))[uM.s-1] Km (1/2 max) (Km): 0.5[uM] max reaction rate (Vmax): (kcat * PKA_cell)[uM.s-1] user defined (kcat): 9.0[s-1]
MAPKK_active_cellMAPKK_Ser_cell	PP2A_inact_MAPKK_active cell	HMMIrreversible	MAPKK_active_cellMAPKK_Ser_cell
		reaction rate (J): ((Vmax * MAPKK_active_cell) / (Km + MAPKK_active_cell))[uM.s-1] Km (1/2 max) (Km): 15.66[uM] max reaction rate (Vmax): (kcat * PP2A_cell)[uM.s-1] user defined (kcat): 6.0[s-1]
MAPK_active_cellMAPK_Thr_cell	MAPK dephosph by PTP cell	HMMIrreversible	MAPK_active_cellMAPK_Thr_cell
		reaction rate (J): ((Vmax * MAPK_active_cell) / (Km + MAPK_active_cell))[uM.s-1] Km (1/2 max) (Km): 0.5[uM] max reaction rate (Vmax): (kcat * PTPSL_cell)[uM.s-1] user defined (kcat): 1.0[s-1]
G_bg_PM+GRK_cellGRK_Gbg_PM	GRK-Gbg binding PM	MassAction	G_bg_PM+GRK_cellGRK_Gbg_PM
		reaction rate (J): (((Kf * G_bg_PM) * GRK_cell) - (Kr * GRK_Gbg_PM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf): 1.0[s-1.uM-1] reverse rate constant (Kr): 0.25[s-1]
MAPK_Thr_cellMAPK_cell	MKPp_decat_MAPKtyr cell	HMMIrreversible	MAPK_Thr_cellMAPK_cell
		reaction rate (J): ((Vmax * MAPK_Thr_cell) / (Km + MAPK_Thr_cell))[uM.s-1] Km (1/2 max) (Km): 0.067[uM] max reaction rate (Vmax): (kcat * MKP1p_cell)[uM.s-1] user defined (kcat): 1.0[s-1]
inhibitedPKA_cellPKI_cell+PKA_cell	PKA release by PKI cell	MassAction	inhibitedPKA_cellPKI_cell+PKA_cell
		reaction rate (J): ((Kf * inhibitedPKA_cell) - ((Kr * PKI_cell) * PKA_cell))[uM.s-1] forward rate constant (Kf): 1.0E-4[s-1] reverse rate constant (Kr): 0.0[s-1.uM-1]
Rap1_GDP_GM+Epac_cAMP_cellRap1_Epac_GM	8a: Rap1-Epac binding GM	MassAction	Rap1_GDP_GM+Epac_cAMP_cellRap1_Epac_GM
		reaction rate (J): (((Kf_8a__Rap1_Epac_binding * Rap1_GDP_GM) * Epac_cAMP_cell) - (Kr_8a__Rap1_Epac_binding * Rap1_Epac_GM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf_8a__Rap1_Epac_binding): 2.0[s-1.uM-1] reverse rate constant (Kr_8a__Rap1_Epac_binding): 0.8[s-1]
cAMP_cell+Epac_cellEpac_cAMP_cell	Epac-cAMP binding cell	MassAction	cAMP_cell+Epac_cellEpac_cAMP_cell
		reaction rate (J): (((Kf_06__Epac_cAMP_binding * cAMP_cell) * Epac_cell) - (Kr_06__Epac_cAMP_binding * Epac_cAMP_cell))[uM.s-1] forward rate constant (Kf_06__Epac_cAMP_binding): 32.0[s-1.uM-1] reverse rate constant (Kr_06__Epac_cAMP_binding): 96.0[s-1]
CREB_nucleusCREB_Ser_nucleus	PKA_act_CREB nucleus	HMMIrreversible	CREB_nucleusCREB_Ser_nucleus
		reaction rate (J): ((Vmax * CREB_nucleus) / (Km + CREB_nucleus))[uM.s-1] Km (1/2 max) (Km): 17.0[uM] max reaction rate (Vmax): (kcat * PKA_nucleus)[uM.s-1] user defined (kcat): 6.0[s-1]
PTPSL_cellPTPSL_star_cell	PKA_deact_PTPSL cell	HMMIrreversible	PTPSL_cellPTPSL_star_cell
		reaction rate (J): ((Vmax * PTPSL_cell) / (Km + PTPSL_cell))[uM.s-1] Km (1/2 max) (Km): 0.1[uM] max reaction rate (Vmax): (kcat * PKA_cell)[uM.s-1] user defined (kcat): 0.2[s-1]
CREB_nucleusCREB_Ser_nucleus	MSK_act_CREB nucleus	HMMIrreversible	CREB_nucleusCREB_Ser_nucleus
		reaction rate (J): ((Vmax * CREB_nucleus) / (Km + CREB_nucleus))[uM.s-1] Km (1/2 max) (Km): 2.0[uM] max reaction rate (Vmax): (kcat * MSK_active_nucleus)[uM.s-1] user defined (kcat): 1.5[s-1]
cAMP_cellAMP_cell	PDE_highKm deact cAMP cell	HMMIrreversible	cAMP_cellAMP_cell
		reaction rate (J): ((Vmax * cAMP_cell) / (Km + cAMP_cell))[uM.s-1] Km (1/2 max) (Km): 15.0[uM] max reaction rate (Vmax): (kcat * PDE_highKm_cell)[uM.s-1] user defined (kcat): 8.0[s-1]
MSK_active_nucleusMSK_nucleus	MSK_dephos nucleus	MassAction	MSK_active_nucleusMSK_nucleus
		reaction rate (J): ((Kf * MSK_active_nucleus) - (Kr * MSK_nucleus))[uM.s-1] forward rate constant (Kf): 0.04[s-1] reverse rate constant (Kr): 0.0[s-1]
Rap1_GDP_GMRap1_GTP_GM	constit exch+hydrolysis GM	MassAction	Rap1_GDP_GMRap1_GTP_GM
		reaction rate (J): ((Kf * Rap1_GDP_GM) - (Kr * Rap1_GTP_GM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf): 3.0E-6[s-1] reverse rate constant (Kr): 8.5E-5[s-1]
MAPK_thr_nucleusMAPK_nucleus	MKP1p deact MAPKtyr nucleus	HMMIrreversible	MAPK_thr_nucleusMAPK_nucleus
		reaction rate (J): ((Vmax * MAPK_thr_nucleus) / (Km + MAPK_thr_nucleus))[uM.s-1] Km (1/2 max) (Km): 0.0667[uM] max reaction rate (Vmax): (kcat * MKP1p_nucleus)[uM.s-1] user defined (kcat): 1.0[s-1]
CREB_Ser_nucleusCREB_nucleus	PP1A_dephos_CREB nucleus	HMMIrreversible	CREB_Ser_nucleusCREB_nucleus
		reaction rate (J): ((Vmax * CREB_Ser_nucleus) / (Km + CREB_Ser_nucleus))[uM.s-1] Km (1/2 max) (Km): 3.0[uM] max reaction rate (Vmax): (kcatPP1A * PP1A_nucleus)[uM.s-1] user defined (kcatPP1A): 0.4[s-1]
MAPK_active_nucleusMAPK_thr_nucleus	MKP1 deact MAPKact nucleus	HMMIrreversible	MAPK_active_nucleusMAPK_thr_nucleus
		reaction rate (J): ((Vmax * MAPK_active_nucleus) / (Km + MAPK_active_nucleus))[uM.s-1] Km (1/2 max) (Km): 0.0667[uM] max reaction rate (Vmax): (kcat * MKP1_nucleus)[uM.s-1] user defined (kcat): 1.0[s-1]
Gsa_GDP_PM+G_bg_PMGs_trimer_PM	14b: Gs trimerization PM	MassAction	Gsa_GDP_PM+G_bg_PMGs_trimer_PM
		reaction rate (J): (((Kf_14b__Gs_trimerization * Gsa_GDP_PM) * G_bg_PM) - (Kr_14b__Gs_trimerization * Gs_trimer_PM))[molecules.um-2.s-1] inward current density (I): 0.0[pA.um-2] net charge valence (netValence): 0.0[1] forward rate constant (Kf_14b__Gs_trimerization): 0.006[um2.s-1.molecules-1] reverse rate constant (Kr_14b__Gs_trimerization): 0.0[s-1]
MAPK_thr_nucleusMAPK_nucleus	MKP1 deact MAPKtyr nucleus	HMMIrreversible	MAPK_thr_nucleusMAPK_nucleus
		reaction rate (J): ((Vmax * MAPK_thr_nucleus) / (Km + MAPK_thr_nucleus))[uM.s-1] Km (1/2 max) (Km): 0.0667[uM] max reaction rate (Vmax): (kcat * MKP1_nucleus)[uM.s-1] user defined (kcat): 1.0[s-1]
MSK_nucleusMSK_active_nucleus	reaction2 nucleus	HMMIrreversible	MSK_nucleusMSK_active_nucleus
		reaction rate (J): ((Vmax * MSK_nucleus) / (Km + MSK_nucleus))[uM.s-1] Km (1/2 max) (Km): 25.0[uM] max reaction rate (Vmax): (kcat * MAPK_active_nucleus)[uM.s-1] user defined (kcat): 10.0[s-1]
MKP1_cell	MKP1 turnover cell	MassAction	MKP1_cell
		reaction rate (J): (Kf * MKP1_cell)[uM.s-1] forward rate constant (Kf): 3.7E-4[s-1] reverse rate constant (Kr): 0.0[uM.s-1]
MAPK_thr_nucleusMAPK_nucleus	MKP2 deact MAPK tyr nucleus	HMMIrreversible	MAPK_thr_nucleusMAPK_nucleus
		reaction rate (J): ((Vmax * MAPK_thr_nucleus) / (Km + MAPK_thr_nucleus))[uM.s-1] Km (1/2 max) (Km): 0.0667[uM] max reaction rate (Vmax): (kcat * MKP2_nucleus)[uM.s-1] user defined (kcat): 1.0[s-1]
MKP1_nucleusMKP1p_nucleus	MKP1 phosph by MAPKactive_nucleus nucleus	HMMIrreversible	MKP1_nucleusMKP1p_nucleus
		reaction rate (J): ((Vmax * MKP1_nucleus) / (Km + MKP1_nucleus))[uM.s-1] Km (1/2 max) (Km): 25.641[uM] max reaction rate (Vmax): (kcat * MAPK_active_nucleus)[uM.s-1] user defined (kcat): 1.0[s-1]
MKP1p_nucleusMKP1_nucleus	MKP1 dephosph in nucleus nucleus	MassAction	MKP1p_nucleusMKP1_nucleus
		reaction rate (J): ((Kf * MKP1p_nucleus) - (Kr * MKP1_nucleus))[uM.s-1] forward rate constant (Kf): 1.0E-4[s-1] reverse rate constant (Kr): 0.0[s-1]
MAPK_active_cellMAPK_Thr_cell	MKP1_deact_MAPKactive cell	HMMIrreversible	MAPK_active_cellMAPK_Thr_cell
		reaction rate (J): ((Vmax * MAPK_active_cell) / (Km + MAPK_active_cell))[uM.s-1] Km (1/2 max) (Km): 0.0667[uM] max reaction rate (Vmax): (kcat * MKP1_cell)[uM.s-1] user defined (kcat): 1.0[s-1]

Structures See in Dashboard
Name	Type Electrical (Membrane Polarity)
golgi	Compartment (+) (-)

cell	Compartment (+) (-)

ec	Compartment (+) (-)

nucleus	Compartment (+) (-)

GM	Membrane N/A

PM	Membrane N/A

nuclear membrane	Membrane N/A

Species See in Dashboard
Name	Structure	BioNetGen Definition
MSK_active_nucleus	nucleus

AMP_cell	cell

BRaf_cell	cell

MAPK_act_cell	cell

cAMP4_R2C2_cell	cell

cAMP4_R2C_cell	cell

cAMP4_R2_cell	cell

MAPK_nucleus	nucleus

MKP1_nucleus	nucleus

MKP2_nucleus	nucleus

MAPK_thr_nucleus	nucleus

MKP1p_cell	cell

MKP1p_nucleus	nucleus

MKP2_cell	cell

G_bg_PM	PM

Rap1_RGII_GM	GM

Gsa_GDP_PM	PM

beta_isop_PM	PM

PDE_star_cell	cell

isoprenaline_ec	ec

MKP1gene_nucleus	nucleus

MSK_nucleus	nucleus

R2C2_cell	cell

PP2A_cell	cell

MAPK_active_nucleus	nucleus

cAMP_R2C2_cell	cell

Rap1_GDP_GM	GM

beta_PM	PM

Epac_cAMP_cell	cell

beta_P_PM	PM

Rap1_Epac_GM	GM

MKP1_RNA_nucleus	nucleus

cAMP2_R2C2_cell	cell

PKA_cell	cell

MAPKK_Ser_cell	cell

PKI_cell	cell

inhibitedPKA_nucleus	nucleus

inhibitedPKA_cell	cell

Gs_trimer_PM	PM

GRK_Gbg_PM	PM

Gs_a_PM	PM

MAPK_active_cell	cell

Rap1GAP_cell	cell

MAPKK_cell	cell

cAMP3_R2C2_cell	cell

cAMP_cell	cell

PP1_cell	cell

PP1A_nucleus	nucleus

CREB_Ser_nucleus	nucleus

PTPSL_star_cell	cell

Epac_cell	cell

MKP1_RNA_cell	cell

Rap1_BRaf_GM	GM

MAPKK_active_cell	cell

PKI_nucleus	nucleus

GRK_cell	cell

AC_PM	PM

AC_Gsa_PM	PM

PDE_cell	cell

Rap1_GTP_GM	GM

pp2a_nucleus	nucleus

CREB_nucleus	nucleus

PTPSL_cell	cell

MAPK_Thr_cell	cell

PDE_PP_cell	cell

PDE_highKm_cell	cell

GRK_PM	PM

betaAR_Gs_PM	PM

BRaf_star_cell	cell

PKA_nucleus	nucleus

MAPK_cell	cell

ATP_cell	cell

MKP1_cell	cell

Molecules See in Dashboard
Name	Text Annotations	BioNetGen Definition

Observables See in Dashboard
Name	Type Count	BioNetGen Definition

Applications

Applications See in Dashboard
Name	Math Type	Annotation
Compart	Deterministic (ODE) Compartmental	cloned from 'Compart' owned by user eungdamr cloned from 'Compart' owned by user argh

Application - Compart

Specifications(Species) - Compart See in Dashboard
Species	Initial Condition	Diffusion Constant
AMP_cell	1.0E-6 [uM]	0.0 [um2/s]
BRaf_cell	0.2 [uM]	0.0 [um2/s]
MAPK_act_cell	0.0 [uM]	0.0 [um2/s]
cAMP4_R2C2_cell	0.0 [uM]	0.0 [um2/s]
cAMP4_R2C_cell	0.0 [uM]	0.0 [um2/s]
cAMP4_R2_cell	0.0 [uM]	0.0 [um2/s]
MKP1p_cell	0.0 [uM]	0.0 [um2/s]
MKP2_cell	0.001 [uM]	0.0 [um2/s]
PDE_star_cell	0.0 [uM]	0.0 [um2/s]
R2C2_cell	0.2 [uM]	0.0 [um2/s]
PP2A_cell	0.075 [uM]	0.0 [um2/s]
cAMP_R2C2_cell	0.0 [uM]	0.0 [um2/s]
Epac_cAMP_cell	1.0E-6 [uM]	0.0 [um2/s]
cAMP2_R2C2_cell	0.0 [uM]	0.0 [um2/s]
PKA_cell	0.0 [uM]	0.0 [um2/s]
MAPKK_Ser_cell	1.0E-6 [uM]	0.0 [um2/s]
PKI_cell	0.4 [uM]	0.0 [um2/s]
inhibitedPKA_cell	0.0 [uM]	0.0 [um2/s]
MAPK_active_cell	0.0 [uM]	0.0 [um2/s]
Rap1GAP_cell	0.15 [uM]	0.0 [um2/s]
MAPKK_cell	0.18 [uM]	0.0 [um2/s]
cAMP3_R2C2_cell	0.0 [uM]	0.0 [um2/s]
cAMP_cell	1.0E-4 [uM]	0.0 [um2/s]
PP1_cell	0.04 [uM]	0.0 [um2/s]
PTPSL_star_cell	0.0 [uM]	0.0 [um2/s]
Epac_cell	0.16 [uM]	0.0 [um2/s]
MKP1_RNA_cell	4.0E-5 [uM]	0.0 [um2/s]
MAPKK_active_cell	1.0E-6 [uM]	0.0 [um2/s]
GRK_cell	0.045 [uM]	0.0 [um2/s]
PDE_cell	0.4 [uM]	0.0 [um2/s]
PTPSL_cell	0.01 [uM]	0.0 [um2/s]
MAPK_Thr_cell	0.0 [uM]	0.0 [um2/s]
PDE_PP_cell	0.2 [uM]	0.0 [um2/s]
PDE_highKm_cell	0.5 [uM]	0.0 [um2/s]
BRaf_star_cell	0.0 [uM]	0.0 [um2/s]
MAPK_cell	0.263 [uM]	0.0 [um2/s]
ATP_cell	4000.0 [uM]	0.0 [um2/s]
MKP1_cell	3.6E-4 [uM]	0.0 [um2/s]
isoprenaline_ec	10.0 [uM]	0.0 [um2/s]
MSK_active_nucleus	0.0 [uM]	0.0 [um2/s]
MAPK_nucleus	0.233 [uM]	0.0 [um2/s]
MKP1_nucleus	2.2E-4 [uM]	0.0 [um2/s]
MKP2_nucleus	1.0E-4 [uM]	0.0 [um2/s]
MAPK_thr_nucleus	0.0 [uM]	0.0 [um2/s]
MKP1p_nucleus	0.0 [uM]	0.0 [um2/s]
MKP1gene_nucleus	0.2 [uM]	0.0 [um2/s]
MSK_nucleus	0.1 [uM]	0.0 [um2/s]
MAPK_active_nucleus	0.0 [uM]	0.0 [um2/s]
MKP1_RNA_nucleus	6.5E-7 [uM]	0.0 [um2/s]
inhibitedPKA_nucleus	0.0 [uM]	0.0 [um2/s]
PP1A_nucleus	0.04 [uM]	0.0 [um2/s]
CREB_Ser_nucleus	0.0 [uM]	0.0 [um2/s]
PKI_nucleus	0.0 [uM]	0.0 [um2/s]
pp2a_nucleus	0.05 [uM]	0.0 [um2/s]
CREB_nucleus	0.3 [uM]	0.0 [um2/s]
PKA_nucleus	0.0 [uM]	0.0 [um2/s]
Rap1_RGII_GM	0.0 [uM]	0.0 [um2/s]
Rap1_GDP_GM	100.0 [uM]	0.0 [um2/s]
Rap1_Epac_GM	0.0 [uM]	0.0 [um2/s]
Rap1_BRaf_GM	0.0 [uM]	0.0 [um2/s]
Rap1_GTP_GM	0.0 [uM]	0.0 [um2/s]
G_bg_PM	0.0 [uM]	0.0 [um2/s]
Gsa_GDP_PM	1.0E-6 [uM]	0.0 [um2/s]
beta_isop_PM	1.0E-6 [uM]	0.0 [um2/s]
beta_PM	33.0 [uM]	0.0 [um2/s]
beta_P_PM	1.0E-6 [uM]	0.0 [um2/s]
Gs_trimer_PM	100.0 [uM]	0.0 [um2/s]
GRK_Gbg_PM	0.0 [uM]	0.0 [um2/s]
Gs_a_PM	1.0E-6 [uM]	0.0 [um2/s]
AC_PM	100.0 [uM]	0.0 [um2/s]
AC_Gsa_PM	1.0E-6 [uM]	0.0 [um2/s]
GRK_PM	1.0E-6 [uM]	0.0 [um2/s]
betaAR_Gs_PM	0.0 [uM]	0.0 [um2/s]

Specifications(Reaction) - Compart See in Dashboard
Name	Reaction Mapping
04: Gs-AC binding	included
05b: cAMP production	included
cAMP bind R2C2	included
MKP2 deact MAPKact	included
10a: Rap1-RGII binding	included
cAMP_binds_cAMPR2C2	included
Gs GTPase	included
10: Rap1 GTPase	included
PP2A_inact_MAPKKSer	included
MAPKK_act_MAPK	included
MKP_translation	included
reaction1	included
PP2A_dephos_PDEstar	included
GRK PM recruitment	included
PKA inhibition by PKI	included
cAMP_bind_cAMP2R2C2	included
Pp2A_deact_CREB	included
2: ARbeta-Gs binding	included
MAPK_act_MKPtranscription	included
PDEstar_deact_cAMP	included
03: GRK activity	included
Release of C2	included
MKP1 basal transcription	included
reaction0	included
reaction3	included
MKP_decat_MAPKtyr	included
RapRaf_act_MAPKKSer	included
MKP1p turnover	included
MKP1 dephosph	included
MKP1p_deact_MAPKactive	included
02b: Gs activation	included
Rap1_binds_BRaf	included
BRaf_act_MAPKK	included
PP_deact_BRaf	included
PKA_act_BRaf	included
cAMP_binds_A2	included
GRK to cytosol	included
08: Epac exchange	included
Release of C1	included
Rap1raf_act_MAPKK	included
MKP1 phospho by MAPKactive	included
MKP1p turnover nucleus	included
07: PDE	included
MKP1p deact MAPKact	included
MKP1 turnover in nucleus	included
01b: isoprenaline binding	included
PTPSL_dephos	included
MAPKK_act_MAPK_thr	included
Receptor recycling	included
PKA_act_PDE	included
PP2A_inact_MAPKK_active	included
MAPK dephosph by PTP	included
GRK-Gbg binding	included
MKPp_decat_MAPKtyr	included
PKA release by PKI	included
8a: Rap1-Epac binding	included
Epac-cAMP binding	included
PKA_act_CREB	included
PKA_deact_PTPSL	included
MSK_act_CREB	included
PDE_highKm deact cAMP	included
MSK_dephos	included
constit exch+hydrolysis	included
MKP1p deact MAPKtyr	included
PP1A_dephos_CREB	included
MKP1 deact MAPKact	included
14b: Gs trimerization	included
MKP1 deact MAPKtyr	included
reaction2	included
MKP1 turnover	included
MKP2 deact MAPK tyr	included
MKP1 phosph by MAPKactive_nucleus	included
MKP1 dephosph in nucleus	included
MKP1_deact_MAPKactive	included
PKI translocation	included
MKP1p translocation	included
MAPK translocation	included
MKP1 translocation	included
MAPKactive_translocation	included
PKA_translocation	included
inhibited PKA export	included
MKPRNA_export	included

Simulations - Compart See in Dashboard
Name	End Time	Output Options
1) MAPK phospho MSK Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
1) MAPK phospho MSK kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
2) MSK dephospho	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
3) PKA phospho CREB Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
3) PKA phospho CREB kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
4) MSK phospho CREB Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
4) MSK phospho CREB kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
5) PP2A dephosho CREB Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
5) PP2A dephosho CREB kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
6) PP1A dephospho CREB Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
6) PP1A dephospho CREB kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
7) MKP1 gene activation Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
7) MKP1 gene activation kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
8) MKP1 basal transcription	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
9) MAPK_act dephospho by MKP2 Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
9) MAPK_act dephospho by MKP2 kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
10) MAPK_act dephospho by MKP1 Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
10) MAPK_act dephospho by MKP1 kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
11) MAPK_act dephospho by MKP1* Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
11) MAPK_act dephospho by MKP1* kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
12) MAPK_tyr dephospho by MKP2 Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
12) MAPK_tyr dephospho by MKP2 kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
13) MAPK_tyr dephospho by MKP Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
13) MAPK_tyr dephospho by MKP kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
14) MAPK_tyr dephospho by MKP1* Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
14) MAPK_tyr dephospho by MKP1* kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
15) MKP1 phospho by MAPK Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
15) MKP1 phospho by MAPK kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
16) MKP1* dephospho kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
17) MKP1 turnover kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
18) MKP1* turnover kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
19) PKI + PKA kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
19) PKI + PKA kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
20) MKP1 translation kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
21) MEK phospho by Braf* Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
21) MEK phospho by Braf* kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
22) MEK_ser phospho by Braf* Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
22) MEK_ser phospho by Braf* kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
23) MAPK phospho by MEK* Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
23) MAPK phospho by MEK* kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
24) MAPK_tyr phospho by MEK* Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
24) MAPK_tyr phospho by MEK* kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
25) cAMP degradation by PDE Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
25) cAMP degradation by PDE kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
26) cAMP degradation by PDE* Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
26) cAMP degradation by PDE* kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
27) cAMP degradation by PDE_high Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
27) cAMP degradation by PDE_high kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
28) Braf phospho by PKA Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
28) Braf phospho by PKA kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
29) Braf dephospho by PP2A Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
29) Braf dephospho by PP2A kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
30) PTP phospho by PKA Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
30) PTP phospho by PKA kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
31) PTP dephospho by PP1 Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
31) PTP dephospho by PP1 kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
32) MEK* dephospho by PP2A Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
32) MEK* dephospho by PP2A kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
33) MEK_ser dephospho by PP2A Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
33) MEK_ser dephospho by PP2A kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
34) MAPK* dephospho by MKP1 Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
34) MAPK* dephospho by MKP1 kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
35) MAPK_tyr dephospho by MKP1 Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
35) MAPK_tyr dephospho by MKP1 kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
36) MAPK* dephospho by MKP1* Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
36) MAPK* dephospho by MKP1* kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
37) MAPK_tyr dephospho by MKP1* Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
37) MAPK_tyr dephospho by MKP1* kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
38) MAPK* dephospho by MKP2 Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
38) MAPK* dephospho by MKP2 kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
39) MAPK_tyr dephospho by MKP2 Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
39) MAPK_tyr dephospho by MKP2 kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
40) MAPK* dephospho by PTP Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
40) MAPK* dephospho by PTP kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
41) MKP1 phospho by MAPK* cyto Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
41) MKP1 phospho by MAPK* cyto kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
42) MKP1* dephospho cyto kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
43) MKP1 degradation kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
44) MKP1* degradation kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
45) PDE phospho by PKA Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
45) PDE phospho by PKA kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
46) PDE* dephospho Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
46) PDE* dephospho kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
47) R2C2 1 kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
47) R2C2 1 kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
48) R2C2 2 kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
48) R2C2 2 kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
49) R2C2 3 kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
49) R2C2 3 kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
50) R2C2 4 kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
50) R2C2 4 kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
51) PKA release 1 kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
51) PKA release 1 kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
52) PKA release 2 kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
52) PKA release 2 kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
53) PKA release by PKI kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
54) EPAC activated by cAMP kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
54) EPAC activated by cAMP kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
55) MEK phospho by RapRaf Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
55) MEK phospho by RapRaf kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
56) MEK_ser phospho by RapRaf Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
56) MEK_ser phospho by RapRaf kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
57) Braf activation by Rap kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
57) Braf activation by Rap kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
58) RapGDP - RapGTP kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
58) RapGDP - RapGTP kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
59) Rap1 activation by EPAC step 1	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
59) Rap1 activation by EPAC step 2	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
60) Rap1 activation by EPAC step 3	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
61) Rap1 deactivation step 1	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
61) Rap1 deactivation step 2	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
62) Rap1 deactivation step 3	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
63) cAMP production Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
63) cAMP production kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
64) BAR phosphorylation Km	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
64) BAR phosphorylation kcat	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
65) BAR activation by ISO kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
65) BAR activation by ISO kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
66) Gtrimer binds to BAR kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
66) Gtrimer binds to BAR kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
67) Gprotein activation kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
67) Gprotein activation kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
68) GRK recruitment by Gbg kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
68) GRK recruitment by Gbg kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
69) GRK on membrane kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
69) GRK on membrane kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
70) GRK to cytosol kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
71) AC activation kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
71) AC activation kr	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
72) Gs deactivation kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
73) G trimerization kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
74) BAR recycling kf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MAPK* import	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MAPK* export	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MAPK import	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MAPK export	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
PKA import	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
PKA export	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
PKI.PKA export	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
PKI import	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MKP1 mRNA export	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MKP1 import	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MKP1 export	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MKP1* import	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MKP1* export	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
Iso	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
BAR	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
Gs trimer	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
AC	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MAPK	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
R2C2	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
ATP	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MKP1	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
bRaf	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
Rap1GAP	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MEK	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
EPAC	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
PTP	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
PP2A	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
GRK	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
PDE	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MKP2	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
Rap1GDP	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MSK	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
PP1A	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
MKP1 DNA	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
PP2A nucleus	3000.0 [s]	KeepEvery 1 KeepAtMost 1000
CREB	3000.0 [s]	KeepEvery 1 KeepAtMost 1000

Geometry non-spatial1212631236 See in Dashboard
(Origin: 0.0, 0.0, 0.0; Extent: 10.0, 10.0, 10.0[um])
Dimension	Type	Subdomain	Adjacent Subvolumes	Size	Mapped Compartment
	Compartmental	Compartment			nuclear membrane

Global Parameters

Global Parameters See in Dashboard
Name	Expression
kimportPKI	0.001[um.s-1]

Km_Rap1BRaf	0.5[uM]

kcat_Rap1Braf	0.105[s-1]