ModelBricks - Model Print Page

Name - AKAP7_PLB_Binding_2PKA

BioModel ID - 95094548

Reaction Diagram

Physiology

Universal ID's	Text Annotations

Reactions See in Dashboard
Reaction	Name Structure	Kinetics	Depiction
AKAP_PLBAKAP_pPLB	PLB_phosphorylation c0	HMMIrreversible	AKAP_PLBAKAP_pPLB
		reaction rate (J): ((Vmax * AKAP_PLB) / (Km + AKAP_PLB))[uM.s-1] Km (1/2 max) (Km): 12.5[uM] max reaction rate (Vmax): (2.0 * AKAP_PLB * (C / ((2.0 * R2C2) + R2C1 + C)) * kcat_PKA)[uM.s-1] user defined (kcat_PKA): 21.0[s-1]
R2_cAMPR2	R2_cAMP_unloading_PDE c0	HMMIrreversible	R2_cAMPR2
		reaction rate (J): ((Vmax * R2_cAMP) / (Km + R2_cAMP))[uM.s-1] Km (1/2 max) (Km): 2.0[uM] max reaction rate (Vmax): (kcat * PDE)[uM.s-1] user defined (kcat): 0.2[s-1]
R2_cAMPR2+cAMP	R2_cAMP_unloading c0	GeneralKinetics	R2_cAMPR2+cAMP
		reaction rate (J): ((Kf * R2_cAMP) - (Kr * cAMP * R2))[uM.s-1] user defined (Kr): 0.3[s-1.uM-1] user defined (Kf): 1.0E-5[s-1]
AKAP+PLBAKAP_PLB	AKAP_PLb_binding c0	MassAction	AKAP+PLBAKAP_PLB
		reaction rate (J): (((Kf * AKAP) * PLB) - (Kr * AKAP_PLB))[uM.s-1] forward rate constant (Kf): 0.179[s-1.uM-1] reverse rate constant (Kr): 0.0024[s-1]
cAMP	cAMP_degradation c0	HMMIrreversible	cAMP
		reaction rate (J): ((Vmax * cAMP) / (Km + cAMP))[uM.s-1] Km (1/2 max) (Km): 1.3[uM] max reaction rate (Vmax): (kcat_PDE * PDE4)[uM.s-1] user defined (kcat_PDE): 2.0[s-1]
AC+GsGsAC	activate_AC c0	MassAction	AC+GsGsAC
		reaction rate (J): (((Kf * AC) * Gs) - (Kr * GsAC))[uM.s-1] forward rate constant (Kf): (GsF * AC_On)[s-1.uM-1] reverse rate constant (Kr): (0.005 * AC_On)[s-1]
GsACAC+pGs	deactivate_AC c0	HMMIrreversible	GsACAC+pGs
		reaction rate (J): ((Vmax * GsAC) / (Km + GsAC))[uM.s-1] Km (1/2 max) (Km): 10.0[uM] max reaction rate (Vmax): ((kcat_PKA * C) * PKA_AC_Fraction)[uM.s-1] user defined (kcat_PKA): 21.0[s-1]
C+R2R2C2	PKA_holoenzyme_formation c0	GeneralKinetics	C+R2R2C2
		reaction rate (J): ((Kf * C * R2) - (Kr * R2C2))[uM.s-1] user defined (Kr): 1.86E-4[s-1] user defined (Kf): 0.799[s-1.uM-1]
ATPcAMP	produce_cAMP c0	HMMIrreversible	ATPcAMP
		reaction rate (J): ((Vmax * ATP) / (Km + ATP))[uM.s-1] Km (1/2 max) (Km): 315.0[uM] max reaction rate (Vmax): ((kcat_AC * GsAC) + (kcat_AC * AC_baseline))[uM.s-1]
cAMP+R2C2R2C1+C	C1_Release c0	GeneralKinetics	cAMP+R2C2R2C1+C
		reaction rate (J): (((cAMP ^ h) / ((Kd_cAMP ^ h) + (cAMP ^ h))) * R2C2)[uM.s-1] user defined (Kd_cAMP): 2.9[tbd] user defined (h): 1.4[tbd]
pPLBPLB	dephosphorylate_PLB c0	HMMIrreversible	pPLBPLB
		reaction rate (J): ((Vmax * pPLB) / (Km + pPLB))[uM.s-1] Km (1/2 max) (Km): 2.7[uM] max reaction rate (Vmax): (2.5 * PP)[uM.s-1]
cAMP+R2C1R2_cAMP+C	C2_Release c0	GeneralKinetics	cAMP+R2C1R2_cAMP+C
		reaction rate (J): (((cAMP ^ h) / ((Kd_cAMP ^ h) + (cAMP ^ h))) * R2C1)[uM.s-1] user defined (Kd_cAMP): 2.9[tbd] user defined (h): 1.4[tbd]
AKAP+pPLBAKAP_pPLB	AKAP-pPLB_dissociation c0	MassAction	AKAP+pPLBAKAP_pPLB
		reaction rate (J): (((Kf * AKAP) * pPLB) - (Kr * AKAP_pPLB))[uM.s-1] forward rate constant (Kf): 0.179[s-1.uM-1] reverse rate constant (Kr): (0.0024 * nR)[s-1]

Structures See in Dashboard
Name	Type Electrical (Membrane Polarity)
c0	Compartment (+) (-)

Species See in Dashboard
Name	Structure	BioNetGen Definition
R2	c0

AC_baseline	c0

C	c0

AKAP	c0

PDE	c0

GsAC	c0

AKAP_PLB	c0

PLB	c0

AKAP_pPLB	c0

pPLB	c0

PP	c0

ATP	c0

pGs	c0

R2C1	c0

R2C2	c0

PDE4	c0

R2_cAMP	c0

cAMP	c0

AC	c0

Gs	c0

Molecules See in Dashboard
Name	Text Annotations	BioNetGen Definition

Observables See in Dashboard
Name	Type Count	BioNetGen Definition

Applications

Applications See in Dashboard
Name	Math Type	Annotation
PLB 2.0uM	Deterministic (ODE) Compartmental	cloned from 'Copy of Application0' owned by user Marc.Rigatti (copied from Application0)
PLB 0.2uM	Deterministic (ODE) Compartmental	(copied from PLB 2.0uM) cloned from 'Copy of Application0' owned by user Marc.Rigatti (copied from Application0)
PLB 5.0uM	Deterministic (ODE) Compartmental	(copied from PLB 2.0uM) cloned from 'Copy of Application0' owned by user Marc.Rigatti (copied from Application0)
cAMP_PDE_Gs_param	Deterministic (ODE) Compartmental	(copied from PLB 2.0uM) cloned from 'Copy of Application0' owned by user Marc.Rigatti (copied from Application0)
Copy of PLB 0.2uM	Deterministic (ODE) Compartmental	(copied from PLB 0.2uM) (copied from PLB 2.0uM) cloned from 'Copy of Application0' owned by user Marc.Rigatti (copied from Application0)
Copy of PLB 2.0uM	Deterministic (ODE) Compartmental	(copied from Copy of PLB 0.2uM) (copied from PLB 0.2uM) (copied from PLB 2.0uM) cloned from 'Copy of Application0' owned by user Marc.Rigatti (copied from Application0)
PLB 38uM	Deterministic (ODE) Compartmental	(copied from Copy of PLB 2.0uM) (copied from Copy of PLB 0.2uM) (copied from PLB 0.2uM) (copied from PLB 2.0uM) cloned from 'Copy of Application0' owned by user Marc.Rigatti (copied from Application0)

Application - PLB 2.0uM

Specifications(Species) - PLB 2.0uM See in Dashboard
Species	Initial Condition	Diffusion Constant
R2	0.19907870489108428 [uM]	0.0 [um2/s]
AC_baseline	0.2 [uM]	0.0 [um2/s]
C	0.027848889674816232 [uM]	0.0 [um2/s]
AKAP	0.02 [uM]	0.0 [um2/s]
PDE	0.2 [uM]	0.0 [um2/s]
GsAC	0.0 [uM]	0.0 [um2/s]
AKAP_PLB	1.0E-6 [uM]	0.0 [um2/s]
PLB	0.2 [uM]	0.0 [um2/s]
AKAP_pPLB	1.0E-6 [uM]	0.0 [um2/s]
pPLB	1.0E-6 [uM]	0.0 [um2/s]
PP	0.01 [uM]	0.0 [um2/s]
ATP	5000.0 [uM]	0.0 [um2/s]
pGs	0.0 [uM]	0.0 [um2/s]
R2C1	0.05738370344261173 [uM]	0.0 [um2/s]
R2C2	0.0573837034412846 [uM]	0.0 [um2/s]
PDE4	0.2 [uM]	0.0 [um2/s]
R2_cAMP	10.686153888225018 [uM]	0.0 [um2/s]
cAMP	0.49196111583075475 [uM]	0.0 [um2/s]
AC	0.5 [uM]	0.0 [um2/s]
Gs	0.5 [uM]	0.0 [um2/s]

Specifications(Reaction) - PLB 2.0uM See in Dashboard
Name	Reaction Mapping
PLB_phosphorylation	included
R2_cAMP_unloading_PDE	included
R2_cAMP_unloading	included
AKAP_PLb_binding	included
cAMP_degradation	included
activate_AC	included
deactivate_AC	included
PKA_holoenzyme_formation	included
produce_cAMP	included
C1_Release	included
dephosphorylate_PLB	included
C2_Release	included
AKAP-pPLB_dissociation	included

Simulations - PLB 2.0uM See in Dashboard
Name	End Time	Output Options
Simulation1	10000.0 [s]	KeepEvery 1 KeepAtMost 1000

Events - PLB 2.0uM See in Dashboard
Name	Trigger Type	Variable Assignment
AC_Stimulation	on condition: (t > 5000.0)	AC_On --> 1.0

Geometry non-spatial1509593381 See in Dashboard
(Origin: 0.0, 0.0, 0.0; Extent: 10.0, 10.0, 10.0[um])
Dimension	Type	Subdomain	Adjacent Subvolumes	Size	Mapped Compartment
	Compartmental	Compartment			c0

Application - PLB 0.2uM

Specifications(Species) - PLB 0.2uM See in Dashboard
Species	Initial Condition	Diffusion Constant
R2	0.19907870489108428 [uM]	0.0 [um2/s]
AC_baseline	0.2 [uM]	0.0 [um2/s]
C	0.027848889674816232 [uM]	0.0 [um2/s]
AKAP	0.02 [uM]	0.0 [um2/s]
PDE	0.2 [uM]	0.0 [um2/s]
GsAC	0.0 [uM]	0.0 [um2/s]
AKAP_PLB	1.0E-6 [uM]	0.0 [um2/s]
PLB	0.2 [uM]	0.0 [um2/s]
AKAP_pPLB	1.0E-6 [uM]	0.0 [um2/s]
pPLB	1.0E-6 [uM]	0.0 [um2/s]
PP	0.01 [uM]	0.0 [um2/s]
ATP	5000.0 [uM]	0.0 [um2/s]
pGs	0.0 [uM]	0.0 [um2/s]
R2C1	0.05738370344261173 [uM]	0.0 [um2/s]
R2C2	0.0573837034412846 [uM]	0.0 [um2/s]
PDE4	0.2 [uM]	0.0 [um2/s]
R2_cAMP	10.686153888225018 [uM]	0.0 [um2/s]
cAMP	0.49196111583075475 [uM]	0.0 [um2/s]
AC	0.5 [uM]	0.0 [um2/s]
Gs	0.5 [uM]	0.0 [um2/s]

Specifications(Reaction) - PLB 0.2uM See in Dashboard
Name	Reaction Mapping
PLB_phosphorylation	included
R2_cAMP_unloading_PDE	included
R2_cAMP_unloading	included
AKAP_PLb_binding	included
cAMP_degradation	included
activate_AC	included
deactivate_AC	included
PKA_holoenzyme_formation	included
produce_cAMP	included
C1_Release	included
dephosphorylate_PLB	included
C2_Release	included
AKAP-pPLB_dissociation	included

Simulations - PLB 0.2uM See in Dashboard
Name	End Time	Output Options
Simulation7	10000.0 [s]	KeepEvery 1 KeepAtMost 1000

Events - PLB 0.2uM See in Dashboard
Name	Trigger Type	Variable Assignment
AC_Stimulation	on condition: (t > 5000.0)	AC_On --> 1.0

Geometry non-spatial1509593381 See in Dashboard
(Origin: 0.0, 0.0, 0.0; Extent: 10.0, 10.0, 10.0[um])
Dimension	Type	Subdomain	Adjacent Subvolumes	Size	Mapped Compartment
	Compartmental	Compartment			c0

Application - PLB 5.0uM

Specifications(Species) - PLB 5.0uM See in Dashboard
Species	Initial Condition	Diffusion Constant
R2	0.19907870489108428 [uM]	0.0 [um2/s]
AC_baseline	0.2 [uM]	0.0 [um2/s]
C	0.027848889674816232 [uM]	0.0 [um2/s]
AKAP	0.02 [uM]	0.0 [um2/s]
PDE	0.2 [uM]	0.0 [um2/s]
GsAC	0.0 [uM]	0.0 [um2/s]
AKAP_PLB	1.0E-6 [uM]	0.0 [um2/s]
PLB	5.0 [uM]	0.0 [um2/s]
AKAP_pPLB	1.0E-6 [uM]	0.0 [um2/s]
pPLB	1.0E-6 [uM]	0.0 [um2/s]
PP	0.01 [uM]	0.0 [um2/s]
ATP	5000.0 [uM]	0.0 [um2/s]
pGs	0.0 [uM]	0.0 [um2/s]
R2C1	0.05738370344261173 [uM]	0.0 [um2/s]
R2C2	0.0573837034412846 [uM]	0.0 [um2/s]
PDE4	0.2 [uM]	0.0 [um2/s]
R2_cAMP	10.686153888225018 [uM]	0.0 [um2/s]
cAMP	0.49196111583075475 [uM]	0.0 [um2/s]
AC	0.5 [uM]	0.0 [um2/s]
Gs	0.25 [uM]	0.0 [um2/s]

Specifications(Reaction) - PLB 5.0uM See in Dashboard
Name	Reaction Mapping
PLB_phosphorylation	included
R2_cAMP_unloading_PDE	included
R2_cAMP_unloading	included
AKAP_PLb_binding	included
cAMP_degradation	included
activate_AC	included
deactivate_AC	included
PKA_holoenzyme_formation	included
produce_cAMP	included
C1_Release	included
dephosphorylate_PLB	included
C2_Release	included
AKAP-pPLB_dissociation	included

Simulations - PLB 5.0uM See in Dashboard
Name	End Time	Output Options
Simulation13	5000.0 [s]	KeepEvery 1 KeepAtMost 1000
PDE_0.2	7000.0 [s]	KeepEvery 1 KeepAtMost 1000
PDE_0.3	7000.0 [s]	KeepEvery 1 KeepAtMost 1000
PDE_0.4	7000.0 [s]	KeepEvery 1 KeepAtMost 1000
AC_0.15_PDE_0.3	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of PDE_0.4	7000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of AC_0.15_PDE_0.3	10000.0 [s]	KeepEvery 1 KeepAtMost 1000

Events - PLB 5.0uM See in Dashboard
Name	Trigger Type	Variable Assignment
AC_Stimulation	on condition: (t > 5000.0)	AC_On --> 1.0

Geometry non-spatial1509593381 See in Dashboard
(Origin: 0.0, 0.0, 0.0; Extent: 10.0, 10.0, 10.0[um])
Dimension	Type	Subdomain	Adjacent Subvolumes	Size	Mapped Compartment
	Compartmental	Compartment			c0

Application - cAMP_PDE_Gs_param

Specifications(Species) - cAMP_PDE_Gs_param See in Dashboard
Species	Initial Condition	Diffusion Constant
R2	0.19907870489108428 [uM]	0.0 [um2/s]
AC_baseline	0.15 [uM]	0.0 [um2/s]
C	0.027848889674816232 [uM]	0.0 [um2/s]
AKAP	0.02 [uM]	0.0 [um2/s]
PDE	0.2 [uM]	0.0 [um2/s]
GsAC	0.0 [uM]	0.0 [um2/s]
AKAP_PLB	1.0E-6 [uM]	0.0 [um2/s]
PLB	2.0 [uM]	0.0 [um2/s]
AKAP_pPLB	1.0E-6 [uM]	0.0 [um2/s]
pPLB	1.0E-6 [uM]	0.0 [um2/s]
PP	0.01 [uM]	0.0 [um2/s]
ATP	5000.0 [uM]	0.0 [um2/s]
pGs	0.0 [uM]	0.0 [um2/s]
R2C1	0.05738370344261173 [uM]	0.0 [um2/s]
R2C2	0.0573837034412846 [uM]	0.0 [um2/s]
PDE4	0.2 [uM]	0.0 [um2/s]
R2_cAMP	10.686153888225018 [uM]	0.0 [um2/s]
cAMP	0.49196111583075475 [uM]	0.0 [um2/s]
AC	1.0 [uM]	0.0 [um2/s]
Gs	0.25 [uM]	0.0 [um2/s]

Specifications(Reaction) - cAMP_PDE_Gs_param See in Dashboard
Name	Reaction Mapping
PLB_phosphorylation	excluded
R2_cAMP_unloading_PDE	included
R2_cAMP_unloading	included
AKAP_PLb_binding	excluded
cAMP_degradation	included
activate_AC	included
deactivate_AC	included
PKA_holoenzyme_formation	included
produce_cAMP	included
C1_Release	included
dephosphorylate_PLB	excluded
C2_Release	included
AKAP-pPLB_dissociation	excluded

Simulations - cAMP_PDE_Gs_param See in Dashboard
Name	End Time	Output Options
Simulation2	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
AC_0.2	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of Copy of Simulation2	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
AC_0.15	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of AC_0.15	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
AC_0.15 PDE_0.2	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Baseline AC PDE_0.2	4999.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of AC_0.15 PDE_0.2	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of Copy of AC_0.15 PDE_0.2	10000.0 [s]	KeepEvery 1 KeepAtMost 1000

Events - cAMP_PDE_Gs_param See in Dashboard
Name	Trigger Type	Variable Assignment
AC_Stimulation	on condition: (t > 5000.0)	AC_On --> 1.0

Geometry non-spatial1509593381 See in Dashboard
(Origin: 0.0, 0.0, 0.0; Extent: 10.0, 10.0, 10.0[um])
Dimension	Type	Subdomain	Adjacent Subvolumes	Size	Mapped Compartment
	Compartmental	Compartment			c0

Application - Copy of PLB 0.2uM

Specifications(Species) - Copy of PLB 0.2uM See in Dashboard
Species	Initial Condition	Diffusion Constant
R2	0.19907870489108428 [uM]	0.0 [um2/s]
AC_baseline	0.15 [uM]	0.0 [um2/s]
C	0.027848889674816232 [uM]	0.0 [um2/s]
AKAP	0.02 [uM]	0.0 [um2/s]
PDE	0.2 [uM]	0.0 [um2/s]
GsAC	0.0 [uM]	0.0 [um2/s]
AKAP_PLB	1.0E-6 [uM]	0.0 [um2/s]
PLB	0.2 [uM]	0.0 [um2/s]
AKAP_pPLB	1.0E-6 [uM]	0.0 [um2/s]
pPLB	1.0E-6 [uM]	0.0 [um2/s]
PP	0.01 [uM]	0.0 [um2/s]
ATP	5000.0 [uM]	0.0 [um2/s]
pGs	0.0 [uM]	0.0 [um2/s]
R2C1	0.05738370344261173 [uM]	0.0 [um2/s]
R2C2	0.0573837034412846 [uM]	0.0 [um2/s]
PDE4	0.2 [uM]	0.0 [um2/s]
R2_cAMP	10.686153888225018 [uM]	0.0 [um2/s]
cAMP	0.49196111583075475 [uM]	0.0 [um2/s]
AC	0.5 [uM]	0.0 [um2/s]
Gs	0.75 [uM]	0.0 [um2/s]

Specifications(Reaction) - Copy of PLB 0.2uM See in Dashboard
Name	Reaction Mapping
PLB_phosphorylation	included
R2_cAMP_unloading_PDE	included
R2_cAMP_unloading	included
AKAP_PLb_binding	included
cAMP_degradation	included
activate_AC	included
deactivate_AC	included
PKA_holoenzyme_formation	included
produce_cAMP	included
C1_Release	included
dephosphorylate_PLB	included
C2_Release	included
AKAP-pPLB_dissociation	included

Simulations - Copy of PLB 0.2uM See in Dashboard
Name	End Time	Output Options
Simulation3	10000.0 [s]	KeepEvery 1 KeepAtMost 1000

Events - Copy of PLB 0.2uM See in Dashboard
Name	Trigger Type	Variable Assignment
AC_Stimulation	on condition: (t > 5000.0)	AC_On --> 1.0

Geometry non-spatial1509593381 See in Dashboard
(Origin: 0.0, 0.0, 0.0; Extent: 10.0, 10.0, 10.0[um])
Dimension	Type	Subdomain	Adjacent Subvolumes	Size	Mapped Compartment
	Compartmental	Compartment			c0

Application - Copy of PLB 2.0uM

Specifications(Species) - Copy of PLB 2.0uM See in Dashboard
Species	Initial Condition	Diffusion Constant
R2	0.19907870489108428 [uM]	0.0 [um2/s]
AC_baseline	0.15 [uM]	0.0 [um2/s]
C	0.027848889674816232 [uM]	0.0 [um2/s]
AKAP	0.02 [uM]	0.0 [um2/s]
PDE	0.2 [uM]	0.0 [um2/s]
GsAC	0.0 [uM]	0.0 [um2/s]
AKAP_PLB	1.0E-6 [uM]	0.0 [um2/s]
PLB	2.0 [uM]	0.0 [um2/s]
AKAP_pPLB	1.0E-6 [uM]	0.0 [um2/s]
pPLB	1.0E-6 [uM]	0.0 [um2/s]
PP	0.01 [uM]	0.0 [um2/s]
ATP	5000.0 [uM]	0.0 [um2/s]
pGs	0.0 [uM]	0.0 [um2/s]
R2C1	0.05738370344261173 [uM]	0.0 [um2/s]
R2C2	0.0573837034412846 [uM]	0.0 [um2/s]
PDE4	0.2 [uM]	0.0 [um2/s]
R2_cAMP	10.686153888225018 [uM]	0.0 [um2/s]
cAMP	0.49196111583075475 [uM]	0.0 [um2/s]
AC	0.5 [uM]	0.0 [um2/s]
Gs	0.75 [uM]	0.0 [um2/s]

Specifications(Reaction) - Copy of PLB 2.0uM See in Dashboard
Name	Reaction Mapping
PLB_phosphorylation	included
R2_cAMP_unloading_PDE	included
R2_cAMP_unloading	included
AKAP_PLb_binding	included
cAMP_degradation	included
activate_AC	included
deactivate_AC	included
PKA_holoenzyme_formation	included
produce_cAMP	included
C1_Release	included
dephosphorylate_PLB	included
C2_Release	included
AKAP-pPLB_dissociation	included

Simulations - Copy of PLB 2.0uM See in Dashboard
Name	End Time	Output Options
Simulation4	10000.0 [s]	KeepEvery 1 KeepAtMost 1000

Events - Copy of PLB 2.0uM See in Dashboard
Name	Trigger Type	Variable Assignment
AC_Stimulation	on condition: (t > 5000.0)	AC_On --> 1.0

Geometry non-spatial1509593381 See in Dashboard
(Origin: 0.0, 0.0, 0.0; Extent: 10.0, 10.0, 10.0[um])
Dimension	Type	Subdomain	Adjacent Subvolumes	Size	Mapped Compartment
	Compartmental	Compartment			c0

Application - PLB 38uM

Specifications(Species) - PLB 38uM See in Dashboard
Species	Initial Condition	Diffusion Constant
R2	0.19907870489108428 [uM]	0.0 [um2/s]
AC_baseline	0.15 [uM]	0.0 [um2/s]
C	0.027848889674816232 [uM]	0.0 [um2/s]
AKAP	0.02 [uM]	0.0 [um2/s]
PDE	0.2 [uM]	0.0 [um2/s]
GsAC	0.0 [uM]	0.0 [um2/s]
AKAP_PLB	1.0E-6 [uM]	0.0 [um2/s]
PLB	38.0 [uM]	0.0 [um2/s]
AKAP_pPLB	1.0E-6 [uM]	0.0 [um2/s]
pPLB	1.0E-6 [uM]	0.0 [um2/s]
PP	0.01 [uM]	0.0 [um2/s]
ATP	5000.0 [uM]	0.0 [um2/s]
pGs	0.0 [uM]	0.0 [um2/s]
R2C1	0.05738370344261173 [uM]	0.0 [um2/s]
R2C2	0.0573837034412846 [uM]	0.0 [um2/s]
PDE4	0.2 [uM]	0.0 [um2/s]
R2_cAMP	10.686153888225018 [uM]	0.0 [um2/s]
cAMP	0.49196111583075475 [uM]	0.0 [um2/s]
AC	0.5 [uM]	0.0 [um2/s]
Gs	0.75 [uM]	0.0 [um2/s]

Specifications(Reaction) - PLB 38uM See in Dashboard
Name	Reaction Mapping
PLB_phosphorylation	included
R2_cAMP_unloading_PDE	included
R2_cAMP_unloading	included
AKAP_PLb_binding	included
cAMP_degradation	included
activate_AC	included
deactivate_AC	included
PKA_holoenzyme_formation	included
produce_cAMP	included
C1_Release	included
dephosphorylate_PLB	included
C2_Release	included
AKAP-pPLB_dissociation	included

Simulations - PLB 38uM See in Dashboard
Name	End Time	Output Options
Baseline cAMP low	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Baseline cAMP ~1uM	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Baseline cAMP ~0.62uM	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Baseline cAMP ~0.62uM increased PP	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Baseline cAMP ~0.62uM Km PP 10 vs 2.7	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Baseline cAMP ~0.62uM Km PP 5	10000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of Baseline cAMP low PLB 50	30000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of Baseline cAMP low	30000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of Copy of Baseline cAMP low PLB 50	30000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of Copy of Copy of Baseline cAMP low PLB 50	30000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of Copy of Copy of Copy of Baseline cAMP low PLB 50	30000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of Copy of Copy of Copy of Baseline cAMP low PLB 50 1	30000.0 [s]	KeepEvery 1 KeepAtMost 1000
Copy of Baseline cAMP ~0.62uM	30000.0 [s]	KeepEvery 1 KeepAtMost 1000
Baseline cAMP ~0.62uM Increased PP	30000.0 [s]	KeepEvery 1 KeepAtMost 1000

Events - PLB 38uM See in Dashboard
Name	Trigger Type	Variable Assignment
AC_Stimulation	on condition: (t > 15000.0)	AC_On --> 1.0

Geometry non-spatial1509593381 See in Dashboard
(Origin: 0.0, 0.0, 0.0; Extent: 10.0, 10.0, 10.0[um])
Dimension	Type	Subdomain	Adjacent Subvolumes	Size	Mapped Compartment
	Compartmental	Compartment			c0

Global Parameters

Global Parameters See in Dashboard
Name	Expression
AC_On	0.0[tbd]

kcat_AC	1.4[tbd]

PKA_AC_Fraction	0.05[tbd]

nR	1.0[tbd]

GsF	0.5[tbd]