ModelBricks - Model Print Page

Name - A predictive computational model reveals that GIV/Girdin serves as a tunable valve for EGFR-stimulated Cyclic AMP Signals

BioModel ID - 156134818

Reaction Diagram

Physiology

Universal ID's	Text Annotations

Reactions See in Dashboard
Reaction	Name Structure	Kinetics	Depiction
AC+Gi_GTPAC_inact	AC_inactivation PM	GeneralLumpedKinetics	AC+Gi_GTPAC_inact
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * k1 * AC * Gi_GTP)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 3.14951E17[um2.s-1.umol-1]
ACAC_i	AC_internalization PM	GeneralLumpedKinetics	ACAC_i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kfint * AC) - (krint * AC_i)))[umol.s-1] net charge valence (netValence): 1.0[1]
AC_inactAC+Gi_GDP	AC_unbinding PM	GeneralLumpedKinetics	AC_inactAC+Gi_GDP
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * k1 * AC_inact)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 0.1667[s-1]
AC_i+Gs_GTP_iAC_act	AC_activation intmem	GeneralLumpedKinetics	AC_i+Gs_GTP_iAC_act
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * k1 * AC_i * Gs_GTP_i)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 1.26462E15[um2.s-1.umol-1]
Cdk5+p35_actCdk5_p35_act	Cdk5_activation PM	GeneralLumpedKinetics	Cdk5+p35_actCdk5_p35_act
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((k1 * p35_act * Cdk5) - (k2 * Cdk5_p35_act)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 45.0[litre.s-1.umol-1] user defined (k2): 10.0[s-1]
DAG	DAG_decactivation PM	GeneralLumpedKinetics	DAG
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * k1 * DAG)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 6.7E-4[s-1]
PKC_actPKC	DAG_deactivation_while_bound PM	GeneralLumpedKinetics	PKC_actPKC
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * k1 * PKC_act)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 0.028[s-1]
GIV_GDIGIV	Degradation_GDI Cyto	GeneralLumpedKinetics	GIV_GDIGIV
		lumped reaction rate (LumpedJ): (Cyto * k1 * GIV_GDI)[umol.s-1] user defined (k1): 0.00107286[s-1]
GIV_GEFGIV	Degradation_GEF Cyto	GeneralLumpedKinetics	GIV_GEFGIV
		lumped reaction rate (LumpedJ): (Cyto * k1 * GIV_GEF)[umol.s-1] user defined (k1): 0.0064638[s-1]
EGF_EGFREGF_EGFR_2	Dimerize PM	GeneralLumpedKinetics	EGF_EGFREGF_EGFR_2
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((k1 * pow(EGF_EGFR,2.0)) - (k2 * EGF_EGFR_2)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 3.14951E17[tbd] user defined (k2): 0.1[tbd]
EGF_EGFR_2EGF_EGFR_2_act	Dimerized_receptor_activation PM	GeneralLumpedKinetics	EGF_EGFR_2EGF_EGFR_2_act
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((k1 * EGF_EGFR_2) - (k2 * EGF_EGFR_2_act)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 1.0[s-1] user defined (k2): 0.01[s-1]
EGFi	EGF_deg Endosome	GeneralLumpedKinetics	EGFi
		lumped reaction rate (LumpedJ): (Endosome * ((kbase + (kc * (Gs_GDP_i + Gs_GDI_i + Gs_GDI_b))) * EGFi))[umol.s-1]
EGFRi	EGFR_deg intmem	GeneralLumpedKinetics	EGFRi
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((kbase + (kc * (Gs_GDP_i + Gs_GDI_i + Gs_GDI_b))) * EGFRi))[umol.s-1] net charge valence (netValence): 1.0[1]
EGFRiEGFR	EGFR_to_PM intmem	GeneralLumpedKinetics	EGFRiEGFR
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((k1 * EGFRi) - (k2 * EGFR)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 0.005[s-1] user defined (k2): 5.0E-5[s-1]
GEF_EGFRGIV_GDI+EGF_EGFR_2_act	EGFR_GEF_to_GDI PM	GeneralLumpedKinetics	GEF_EGFRGIV_GDI+EGF_EGFR_2_act
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kcat * PKC_act * GEF_EGFR) / (Km + GEF_EGFR)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 2.10987E-15[umol.um-2] user defined (kcat): 2.53373[s-1]
GIV_GDI+Gs_GDP_iGs_GDI_i	GDI_function intmem	GeneralLumpedKinetics	GIV_GDI+Gs_GDP_iGs_GDI_i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((GDI_function_k1 * Gs_GDP_i * GIV_GDI) - (krGDIGIV * Gs_GDI_i)))[umol.s-1] net charge valence (netValence): 1.0[1]
GIV_GEF+EGF_EGFR_2_actGEF_EGFR	GEF_EGFR_binding PM	GeneralLumpedKinetics	GIV_GEF+EGF_EGFR_2_actGEF_EGFR
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((k1 * EGF_EGFR_2_act * GIV_GEF) - (k2 * GEF_EGFR)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 0.00145[litre.s-1.umol-1] user defined (k2): 1.09025E-7[s-1]
GEF_EGFR+Gi_GDPGEF_Gi_EGFR	GEF_Gi_Binding PM	GeneralLumpedKinetics	GEF_EGFR+Gi_GDPGEF_Gi_EGFR
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kfGi * GEF_EGFR * Gi_GDP) - (krGi * GEF_Gi_EGFR)))[umol.s-1] net charge valence (netValence): 1.0[1]
GIVGIV_GEF	GEF_activation PM	GeneralLumpedKinetics	GIVGIV_GEF
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kcat * Cdk5_p35_act * GIV) / (Km + GIV)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 6.0[umol.litre-1] user defined (kcat): 0.6[s-1]
GIV_GEFGIV_GDI	GEF_to_GDI PM	GeneralLumpedKinetics	GIV_GEFGIV_GDI
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kcat * PKC_act * GIV_GEF) / (Km + GIV_GEF)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 6.0[umol.litre-1] user defined (kcat): 0.999995[s-1]
GEF_Gi_EGFRGEF_EGFR+Gi_GTP	Gi_activation PM	GeneralLumpedKinetics	GEF_Gi_EGFRGEF_EGFR+Gi_GTP
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * k1 * GEF_Gi_EGFR)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 14.4682[s-1]
Gi_GTPGi_GDP	Gi_hydrolysis PM	GeneralLumpedKinetics	Gi_GTPGi_GDP
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * k1 * Gi_GTP)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 0.139[s-1]
EGF_EGFR_i_act	Gs_GDI_accel_deg intmem	GeneralLumpedKinetics	EGF_EGFR_i_act
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((kbase + (kc * (Gs_GDP_i + Gs_GDI_i + Gs_GDI_b))) * EGF_EGFR_i_act))[umol.s-1] net charge valence (netValence): 1.0[1]
Gs_protein_ibg_i+Gs_GTP_i	Gs_activation intmem	GeneralLumpedKinetics	Gs_protein_ibg_i+Gs_GTP_i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((kcat * EGF_EGFR_i_act * Gs_protein_i) / (Km + Gs_protein_i)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 5.0E-15[umol.um-2] user defined (kcat): 0.4[s-1]
Gs_GTP_iGs_GDP_i	Gs_hydrolysis intmem	GeneralLumpedKinetics	Gs_GTP_iGs_GDP_i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * k1 * Gs_GTP_i)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 0.139[s-1]
Gs_proteinGs_protein_i	Gs_internalization PM	GeneralLumpedKinetics	Gs_proteinGs_protein_i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kfint * Gs_protein) - (krint * Gs_protein_i)))[umol.s-1] net charge valence (netValence): 1.0[1]
IP3inositol	IP3_degradation Cyto	GeneralLumpedKinetics	IP3inositol
		lumped reaction rate (LumpedJ): (Cyto * k1 * IP3)[umol.s-1] user defined (k1): 2.5[s-1]
AC_actAC_i+Gs_GDP_i	InactivAC intmem	GeneralLumpedKinetics	AC_actAC_i+Gs_GDP_i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * k1 * AC_act)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 0.1667[s-1]
PDEPDEp	PDE_phos Cyto	GeneralLumpedKinetics	PDEPDEp
		lumped reaction rate (LumpedJ): (Cyto * ((kcat * PKA * PDE) / (Km + PDE)))[umol.s-1] user defined (Km): 0.5[umol.litre-1] user defined (kcat): 5.0[s-1]
PIP2DAG+IP3	PIP2_to_DAG PM	GeneralLumpedKinetics	PIP2DAG+IP3
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kcat * PLCg_active * PIP2) / (Km + PIP2)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 8.3E-15[umol.um-2] user defined (kcat): 2.3E-17[litre.s-1.um-2]
PKAPKA_inact	PKA_inactivation Cyto	GeneralLumpedKinetics	PKAPKA_inact
		lumped reaction rate (LumpedJ): (Cyto * ((k1 * PKA) - (k2 * PKA_inact)))[umol.s-1] user defined (k1): 1.0E-4[s-1] user defined (k2): 1.0[s-1]
R2uu+PKAR2C2	PKA_reformation Cyto	GeneralLumpedKinetics	R2uu+PKAR2C2
		lumped reaction rate (LumpedJ): (Cyto * k1 * R2uu * pow(PKA,2.0))[umol.s-1] user defined (k1): 10.0[tbd]
PKC+DAGPKC_act	PKC_activation PM	GeneralLumpedKinetics	PKC+DAGPKC_act
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((k1 * DAG * PKC) - (k2 * PKC_act)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 0.1[litre.s-1.umol-1] user defined (k2): 1.0E-5[s-1]
PLCgPLCg_active	PLC_GEF_enhanced PM	GeneralLumpedKinetics	PLCgPLCg_active
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kcat * GEF_EGFR * PLCg) / (Km + PLCg)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 1.0[umol.litre-1] user defined (kcat): 0.103589[s-1]
PLCgPLCg_active	PLC_activation_basal PM	GeneralLumpedKinetics	PLCgPLCg_active
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kcat * EGF_EGFR_2_act * PLCg) / (Km + PLCg)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 1.0[umol.litre-1] user defined (kcat): 1.00002E-4[s-1]
PLCg_activePLCg	PLC_deactivation Cyto	GeneralLumpedKinetics	PLCg_activePLCg
		lumped reaction rate (LumpedJ): (Cyto * k1 * PLCg_active)[umol.s-1] user defined (k1): 1.00002E-5[s-1]
R2uR2uu+cAMP	R2uUnbind Cyto	GeneralLumpedKinetics	R2uR2uu+cAMP
		lumped reaction rate (LumpedJ): (Cyto * k1 * R2u)[umol.s-1] user defined (k1): 2.78E-4[s-1]
R2R2u+cAMP	R2Unbind Cyto	GeneralLumpedKinetics	R2R2u+cAMP
		lumped reaction rate (LumpedJ): (Cyto * k1 * R2)[umol.s-1] user defined (k1): 0.0167[s-1]
R2C2+cAMPR2+PKA	cAMP_Activation Cyto	GeneralLumpedKinetics	R2C2+cAMPR2+PKA
		lumped reaction rate (LumpedJ): (Cyto * ((V * R2C2 * pow((cAMP / Shalve),h)) / (1.0 + pow((cAMP / Shalve),h))))[umol.s-1] user defined (V): 0.05[s-1] user defined (Shalve): 0.54[umol.litre-1] user defined (h): 1.75[1]
ATPcAMP	cAMP_basal PM	GeneralLumpedKinetics	ATPcAMP
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kcat * AC * ATP) / (Km + ATP)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 1030.0[umol.litre-1] user defined (kcat): 0.12[s-1]
ATPcAMP	cAMP_basal_production_internal intmem	GeneralLumpedKinetics	ATPcAMP
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((kcat * AC_i * ATP) / (Km + ATP)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 1030.0[umol.litre-1] user defined (kcat): 0.12[s-1]
cAMPAMP	cAMP_to_AMP Cyto	GeneralLumpedKinetics	cAMPAMP
		lumped reaction rate (LumpedJ): (Cyto * ((kcat * PDE * cAMP) / (Km + cAMP)))[umol.s-1] user defined (Km): 1.51[umol.litre-1] user defined (kcat): 2.0[s-1]
cAMPAMP	cAMP_to_AMP_PDEp Cyto	GeneralLumpedKinetics	cAMPAMP
		lumped reaction rate (LumpedJ): (Cyto * ((kcat * PDEp * cAMP) / (Km + cAMP)))[umol.s-1] user defined (Km): 1.26[umol.litre-1] user defined (kcat): 5.0[s-1]
PDEpPDE	dephos_of_PDEp Cyto	GeneralLumpedKinetics	PDEpPDE
		lumped reaction rate (LumpedJ): (Cyto * ((kcat * PP_PDE * PDEp) / (Km + PDEp)))[umol.s-1] user defined (Km): 8.0[umol.litre-1] user defined (kcat): 10.0[s-1]
EGF_EGFR_2_actEGF_EGFR_i_act	internal_transport PM	GeneralLumpedKinetics	EGF_EGFR_2_actEGF_EGFR_i_act
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * intrec * EGF_EGFR_2_act)[umol.s-1] net charge valence (netValence): 1.0[1]
bg_i+Gs_GDP_iGs_protein_i	internal_trimerize intmem	GeneralLumpedKinetics	bg_i+Gs_GDP_iGs_protein_i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * k1 * bg_i * Gs_GDP_i)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 8.91256E14[um2.s-1.umol-1]
p35p35_act	p35_activation PM	GeneralLumpedKinetics	p35p35_act
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((kcat * EGF_EGFR_2_act * p35) / (Km + p35)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 1.589E-15[umol.um-2] user defined (kcat): 0.1[s-1]
p35_actp35	p35_inactivation PM	GeneralLumpedKinetics	p35_actp35
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * k1 * p35_act)[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 0.001[s-1]
ATPcAMP	production_cAMP_internal intmem	GeneralLumpedKinetics	ATPcAMP
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((kcat * AC_act * ATP) / (Km + ATP)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (Km): 315.0[umol.litre-1] user defined (kcat): 13.0[s-1]
EGF+EGFREGF_EGFR	receptor_ligand_binding PM	GeneralLumpedKinetics	EGF+EGFREGF_EGFR
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (PM * ((k1 * EGFR * EGF) - (k2 * EGF_EGFR)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 3.0[litre.s-1.umol-1] user defined (k2): 0.014[s-1]
EGFi+EGFRiEGF_EGFRi	v10 intmem	GeneralLumpedKinetics	EGFi+EGFRiEGF_EGFRi
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((k1 * EGFRi * EGFi) - (k2 * EGF_EGFRi)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 0.14[litre.s-1.umol-1] user defined (k2): 0.0195[s-1]
EGF_EGFRiEGF_EGFR_2i	v11 intmem	GeneralLumpedKinetics	EGF_EGFRiEGF_EGFR_2i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((k1 * pow(EGF_EGFRi,2.0)) - (k2 * EGF_EGFR_2i)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 4.0E-5[tbd] user defined (k2): 0.1[tbd]
EGF_EGFR_2iEGF_EGFR_i_act	v12 intmem	GeneralLumpedKinetics	EGF_EGFR_2iEGF_EGFR_i_act
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((k1 * EGF_EGFR_2i) - (k2 * EGF_EGFR_i_act)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (k1): 1.0[s-1] user defined (k2): 0.01[s-1]
GIV+Gs_GDP_iGs_GDI_b	basalGIVGDI Cyto	GeneralLumpedKinetics	GIV+Gs_GDP_iGs_GDI_b
		lumped reaction rate (LumpedJ): (Cyto * ((bindingGDIGIV * GIV * Gs_GDP_i) - (krGDIGIV * Gs_GDI_b)))[umol.s-1]
GIV89pGIV	_89pDEG Cyto	GeneralLumpedKinetics	GIV89pGIV
		lumped reaction rate (LumpedJ): (Cyto * k1 * GIV89p)[umol.s-1] user defined (k1): 0.01[s-1]
Gs_GDI_i	Gs_GDI_accel_deg_copy intmem	GeneralLumpedKinetics	Gs_GDI_i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * (I * Gs_GDI_i))[umol.s-1] net charge valence (netValence): 1.0[1]
Gs_GDP_i	Gs_GDI_accel_deg_copy2 intmem	GeneralLumpedKinetics	Gs_GDP_i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * (Gs_GDP_i * Gs_GDP_i))[umol.s-1] net charge valence (netValence): 1.0[1]
Gs_GTP_i	Gs_GDI_accel_deg_copy3 intmem	GeneralLumpedKinetics	Gs_GTP_i
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * (Gs_GDP_i * Gs_GTP_i))[umol.s-1] net charge valence (netValence): 1.0[1]
AC_act	Gs_GDI_accel_deg_copy4 intmem	GeneralLumpedKinetics	AC_act
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((kbase + (kc * (Gs_GDP_i + Gs_GDI_i + Gs_GDI_b))) * AC_act))[umol.s-1] net charge valence (netValence): 1.0[1]
EGF_EGFRi	Gs_GDI_accel_deg_copy5 intmem	GeneralLumpedKinetics	EGF_EGFRi
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * ((kbase + (kc * (Gs_GDP_i + Gs_GDI_i + Gs_GDI_b))) * EGF_EGFRi))[umol.s-1] net charge valence (netValence): 1.0[1]
Gs_GDI_b	Gs_GDI_accel_deg_copy6 intmem	GeneralLumpedKinetics	Gs_GDI_b
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * (I * Gs_GDI_b))[umol.s-1] net charge valence (netValence): 1.0[1]
I	Intfun intmem	GeneralLumpedKinetics	I
		lumped current (LumpedI): 0.0[pA] lumped reaction rate (LumpedJ): (intmem * (kc * (Gs_GDP_i + Gs_GDI_i + Gs_GDI_b)))[umol.s-1] net charge valence (netValence): 1.0[1] user defined (kc): 4.2968E7[s-1]

Structures See in Dashboard
Name	Type Electrical (Membrane Polarity)
Endosome	Compartment (+) (-)

Cyto	Compartment (+) (-)

ECM	Compartment (+) (-)

intmem	Membrane N/A

PM	Membrane N/A

Species See in Dashboard
Name	Structure	BioNetGen Definition
EGF	ECM

EGFR	PM

p35	PM

p35_act	PM

Gi_GTP	PM

Gi_GDP	PM

EGF_EGFR	PM

PLCg	Cyto

IP3	Cyto

inositol	Cyto

PKC	Cyto

PKC_act	PM

AC	PM

AC_act	intmem

ATP	Cyto

cAMP	Cyto

PKA	Cyto

PDE	Cyto

R2C2	Cyto

AMP	Cyto

PDEp	Cyto

PIP2	PM

EGF_EGFR_2_act	PM

EGF_EGFR_2	PM

PP_PDE	Cyto

DAG	PM

PLCg_active	Cyto

GEF_EGFR	PM

GEF_Gi_EGFR	PM

GIV	Cyto

GIV_GEF	Cyto

GIV_GDI	Cyto

Cdk5	Cyto

Gs_GTP_i	intmem

Gs_GDP_i	intmem

Gs_GDI_i	intmem

EGF_EGFR_i_act	intmem

AC_i	intmem

bg_i	intmem

AC_inact	PM

Gs_protein	PM

EGFi	Endosome

EGFRi	intmem

R2	Cyto

R2u	Cyto

R2uu	Cyto

EGF_EGFRi	intmem

EGF_EGFR_2i	intmem

Cdk5_p35_act	PM

Gs_protein_i	intmem

PKA_inact	Cyto

Gs_GDI_b	intmem

GIV89p	Cyto

I	intmem

Molecules See in Dashboard
Name	Text Annotations	BioNetGen Definition

Observables See in Dashboard
Name	Type Count	BioNetGen Definition

Applications

Applications See in Dashboard
Name	Math Type	Annotation
unnamed	Deterministic (ODE) Compartmental

Application - unnamed

Specifications(Species) - unnamed See in Dashboard
Species	Initial Condition	Diffusion Constant
EGFi	0.0 [umol]	0.0 [um2/s]
PLCg	0.1 [umol]	0.0 [um2/s]
IP3	0.0 [umol]	0.0 [um2/s]
inositol	0.0 [umol]	0.0 [um2/s]
PKC	0.1 [umol]	0.0 [um2/s]
ATP	5000.0 [umol]	0.0 [um2/s]
cAMP	0.00463445029560112 [umol]	0.0 [um2/s]
PKA	0.00663502149436935 [umol]	0.0 [um2/s]
PDE	0.3454122887 [umol]	0.0 [um2/s]
R2C2	0.0963501127833185 [umol]	0.0 [um2/s]
AMP	1000.0 [umol]	0.0 [um2/s]
PDEp	0.0545877112954137 [umol]	0.0 [um2/s]
PP_PDE	0.2 [umol]	0.0 [um2/s]
PLCg_active	0.0 [umol]	0.0 [um2/s]
GIV	((totalGIV - (GDImult * totalGIV)) - GIV_GEF) [umol]	0.0 [um2/s]
GIV_GEF	(GEFmult * totalGIV) [umol]	0.0 [um2/s]
GIV_GDI	0.0 [umol]	0.0 [um2/s]
Cdk5	0.05 [umol]	0.0 [um2/s]
R2	6.97924584116095E-5 [umol]	0.0 [um2/s]
R2u	0.00419256840426028 [umol]	0.0 [um2/s]
R2uu	0.00264752635400966 [umol]	0.0 [um2/s]
PKA_inact	0.0 [umol]	0.0 [um2/s]
GIV89p	(GDImult * totalGIV) [umol]	0.0 [um2/s]
EGF	0.05 [umol]	0.0 [um2/s]
AC_act	0.0 [umol]	0.0 [um2/s]
Gs_GTP_i	0.0 [umol]	0.0 [um2/s]
Gs_GDP_i	0.0 [umol]	0.0 [um2/s]
Gs_GDI_i	0.0 [umol]	0.0 [um2/s]
EGF_EGFR_i_act	0.0 [umol]	0.0 [um2/s]
AC_i	0.0 [umol]	0.0 [um2/s]
bg_i	0.0 [umol]	0.0 [um2/s]
EGFRi	0.0 [umol]	0.0 [um2/s]
EGF_EGFRi	0.0 [umol]	0.0 [um2/s]
EGF_EGFR_2i	0.0 [umol]	0.0 [um2/s]
Gs_protein_i	0.0 [umol]	0.0 [um2/s]
Gs_GDI_b	1.30617460365262E-15 [umol]	0.0 [um2/s]
I	0.0 [umol]	0.0 [um2/s]
EGFR	3.985E-16 [umol]	0.0 [um2/s]
p35	1.589E-15 [umol]	0.0 [um2/s]
p35_act	0.0 [umol]	0.0 [um2/s]
Gi_GTP	0.0 [umol]	0.0 [um2/s]
Gi_GDP	7.97E-17 [umol]	0.0 [um2/s]
EGF_EGFR	0.0 [umol]	0.0 [um2/s]
PKC_act	0.0 [umol]	0.0 [um2/s]
AC	4.98E-17 [umol]	0.0 [um2/s]
PIP2	1.589E-14 [umol]	0.0 [um2/s]
EGF_EGFR_2_act	0.0 [umol]	0.0 [um2/s]
EGF_EGFR_2	0.0 [umol]	0.0 [um2/s]
DAG	0.0 [umol]	0.0 [um2/s]
GEF_EGFR	0.0 [umol]	0.0 [um2/s]
GEF_Gi_EGFR	2.0E-20 [umol]	0.0 [um2/s]
AC_inact	0.0 [umol]	0.0 [um2/s]
Gs_protein	2.49E-15 [umol]	0.0 [um2/s]
Cdk5_p35_act	0.0 [umol]	0.0 [um2/s]

Specifications(Reaction) - unnamed See in Dashboard
Name	Reaction Mapping
AC_inactivation	included
AC_internalization	included
AC_unbinding	included
AC_activation	included
Cdk5_activation	included
DAG_decactivation	included
DAG_deactivation_while_bound	included
Degradation_GDI	included
Degradation_GEF	included
Dimerize	included
Dimerized_receptor_activation	included
EGF_deg	included
EGFR_deg	included
EGFR_to_PM	included
EGFR_GEF_to_GDI	included
GDI_function	included
GEF_EGFR_binding	included
GEF_Gi_Binding	included
GEF_activation	included
GEF_to_GDI	included
Gi_activation	included
Gi_hydrolysis	included
Gs_GDI_accel_deg	included
Gs_activation	included
Gs_hydrolysis	included
Gs_internalization	included
IP3_degradation	included
InactivAC	included
PDE_phos	included
PIP2_to_DAG	included
PKA_inactivation	included
PKA_reformation	included
PKC_activation	included
PLC_GEF_enhanced	included
PLC_activation_basal	included
PLC_deactivation	included
R2uUnbind	included
R2Unbind	included
cAMP_Activation	included
cAMP_basal	included
cAMP_basal_production_internal	included
cAMP_to_AMP	included
cAMP_to_AMP_PDEp	included
dephos_of_PDEp	included
internal_transport	included
internal_trimerize	included
p35_activation	included
p35_inactivation	included
production_cAMP_internal	included
receptor_ligand_binding	included
v10	included
v11	included
v12	included
basalGIVGDI	included
_89pDEG	included
Gs_GDI_accel_deg_copy	included
Gs_GDI_accel_deg_copy2	included
Gs_GDI_accel_deg_copy3	included
Gs_GDI_accel_deg_copy4	included
Gs_GDI_accel_deg_copy5	included
Gs_GDI_accel_deg_copy6	included
Intfun	included

Simulations - unnamed See in Dashboard
Name	End Time	Output Options
Simulation0	3600.0 [s]	OutputTimeStep 5.0
GIV+EGFR variation	3600.0 [s]	OutputTimeStep 5.0

Events - unnamed See in Dashboard
Name	Trigger Type	Variable Assignment
EGFoff	on condition: 0.0	EGF --> 0.0

Geometry Compartmental See in Dashboard
(Origin: 0.0, 0.0, 0.0; Extent: 10.0, 10.0, 10.0[um])
Dimension	Type	Subdomain	Adjacent Subvolumes	Size	Mapped Compartment
	Compartmental	Compartment			PM

Global Parameters

Global Parameters See in Dashboard
Name	Expression
kfint	4.578940789E-5[tbd]

krint	6.66502822995389E-5[tbd]

kbase	1.07E-8[tbd]

kc	1.32E11[tbd]

kfGi	3.0102889041494098E18[tbd]

krGi	((0.24 * ModelValue_4 * Compartment_2) / Compartment_1)[tbd]

intrec	0.002[tbd]

normcAMP	(cAMP / Metabolite_15)[tbd]

normGDI	((Gs_GDI_i + Gs_GDI_b) / (Metabolite_35 + Metabolite_51))[tbd]

normGEF	(GEF_Gi_EGFR / Metabolite_28)[tbd]

normGEFc	((GIV_GEF + GIV_GDI) / (Metabolite_30 + Metabolite_31))[tbd]

normGDIc	((GIV_GDI + GIV89p) / (Metabolite_31 + Metabolite_52))[tbd]

totalGIV	1.0[tbd]

GDImult	(2.0 * GEFmult)[tbd]

bindingGDIGIV	(0.05 * GDI_function_k1)[tbd]

krGDIGIV	3.0E-4[tbd]

GEFmult	0.0511120195467634[tbd]

GDI_function_k1	2.91[tbd]

Compartment_2	Cyto[tbd]

Metabolite_52	GIV89p[tbd]

Metabolite_31	GIV_GDI[tbd]

Metabolite_30	GIV_GEF[tbd]

Metabolite_15	cAMP[tbd]

Compartment_1	PM[tbd]

Metabolite_28	GEF_Gi_EGFR[tbd]

Metabolite_51	Gs_GDI_b[tbd]

Metabolite_35	Gs_GDI_i[tbd]

ModelValue_4	kfGi[tbd]